肿瘤特异性Thl细胞联合脂质体多肽抗肿瘤活性研究  

Anti - cancer activity of tumor - specific Thl cell combined with lipsome - peptides

作　　者：张悦[1] 张清媛[1] 赵红[1] 汤大北[1] 甄丽娜[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院肿瘤内科,哈尔滨150081

出　　处：《实用肿瘤学杂志》2013年第5期391-395,共5页Practical Oncology Journal

基　　金：高等学校博士学科点专项科研基金（20092307120011）;黑龙江省留学归国科学基金项目（LC2009C23）;哈尔滨市科技创新人才研究专项资金项目（2010RPLXS019）

摘　　要：目的探讨脂质体多肽复合物联合Thl细胞的抗肿瘤活性及机制。方法建立荷黑色素小鼠模型，实验分为对照组、Thl＋多肽治疗组、Thl＋脂质体多肽治疗组以及Thl＋OVA治疗组。观察各组别对肿瘤体积、宿主生存及淋巴细胞活化能力的影响。结果与对照组及Thl＋多肽治疗组相比，Thl＋脂质体多肽治疗组可显著抑制肿瘤的生长，延长荷瘤小鼠生存时间，并通过活化肿瘤特异性细胞毒性T细胞提高抗肿瘤活性。结论Thl联合脂质体多肽复合物免疫疗法可有效诱导荷瘤机体产生有效的抗肿瘤活性。Objeeive The present study aims to assess the anti - tumor effects of combined treatment with liposome -peptides with Thl cells and to investigate the possible involved mechanism. Methods B16 - OVA melanoma tumor - bearing mice model was established. The mice were divided into the control group ,Thl ＋ peptides group,Thl ＋ liposome -peptides group and Thl ＋ OVA group. The changes in tumor volume, survival of recipients and activities of immune cells were analyzed. Results We found that tumor growth was significantly inhibited and survival rate was significantly improved by tile treatment with Thl ＋ liposome - peptides as com- pared with contol group and Thl ＋ peptides group. Tumor eradication was crucially dependent on the generation of tumor - specific CTLs in the tumor DLNs and tumor site. Conclusion These results identify that Thl combined with liposome -peptides is a novel approach to induce anti -tumor immune effectively.

关 键 词：肿瘤治疗学 肿瘤特异性Th1细胞 脂质体 多肽 

分 类 号：R750.5[医药卫生—皮肤病学与性病学]