银杏叶脂溶性不皂化物轻馏分对促进黄帝菊种子萌发和幼苗生长的影响  

Effects of Light Distillates Separated from Fat-soluble Unsaponifiable Matter of Ginkgo biloba Leaves on Melampodium paludosum Seed Germination and Seedling Growth

作　　者：陈虹霞[1,2,3,4] 王成章[1,2,3,4,5] 孙燕[1,2,3,4] 陶冉[1,2,3,4] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042 [5]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《林产化学与工业》2013年第5期50-54,共5页Chemistry and Industry of Forest Products

基　　金：'十二五'国家科技支撑计划资助(2011BAD33B00);林业公益性行业科研专项(201004007);国家自然科学基金资助项目(30871986)

摘　　要：选用黄帝菊为供试材料,研究了不同浓度银杏叶脂溶性不皂化物轻馏分对种子萌发和幼苗生长的影响。结果表明,50 mg/L的轻馏分提取物能够促进黄帝菊种子的萌发和幼苗的生长,发芽率为73%,苗高和根长分别为2.92和4.87 cm,分别为对照组的122%、127%和123%,促进效果显著。轻馏分处理能明显提高种子淀粉酶活性以及幼苗的叶绿素质量分数、可溶性蛋白含量和根系活力。轻馏分为50 mg/L时,淀粉酶活性为0.72 mg/(g·min),根系活力为58.18μg/(g·h),幼苗叶绿素质量分数1.679 mg/g,幼苗可溶性蛋白0.061 mg/g,分别为对照组的150%、136%、183%和161%。Effects of the light distillates from fat-soluble unsaponifiable matter of Ginkgo biloba L. leaves with different concentrations on Melampodium paludosum Kunth seed germination and seedlings growth were studied. The results indicated that ： the concentrations （50 mg/L） of the light distillates has high hastened effect on seed germination and seedling growth. The germination rate was 73 %. The seedling height and root length of M. paludosum were 2.92 and 4.87 cm, were 122 %, 127 % and 123 % of the control group, respectively. The promotion effect is remarkable. The contents of chlorophyll, soluble protein in seedlings and activities of root, amylase of seeds increased after treated with light distillates. When the light distillates was 50 rag/L, it was found that the amylase activity was 0.72 mg/（ g. rain）, the root activity was 58.18 p.g/（ g- h）, and the contents of chlorophyll and solution protein were l. 679 mg/g and 0. 061 rag/g, respectively. They were 150 %, 136 %, 183 % and 161% of the control group, respectively.

关 键 词：银杏叶 不皂化物 轻馏分 黄帝菊 种子萌发 幼苗生长 

分 类 号：TQ35[化学工程]