藏红花素对耐伊马替尼慢性白血病粒细胞的促凋亡作用  

Apoptotic Induction Effect and Mechanism of Crocin on Imatinib Resistant K562 / Ima Cells

作　　者：张洋[1] 郑卫东[1] 邸玉玮[1] 方伟[1] 廖玉琴[1] 

机构地区：[1]广东省医学科学院//广东省人民医院检验科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2013年第4期537-541,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金博士启动项目(2008)(8451008004001345)

摘　　要：【目的】分析探讨藏红花素对耐伊马替尼白血病细胞株K562/Ima的增殖抑制和促凋亡的机制。【方法】耐伊马替尼的白血病细胞株K562/Ima接种于96孔板中,经不同浓度的藏红花素分别处理48h采用CCK8法检测细胞增殖抑制情况,Hoechst染色藏红花素处理的细胞,荧光显微镜观察及采用Annexin V/PI双染分析藏红花素对K562/Ima细胞的促凋亡作用。K562/Ima细胞经藏红花素处理,及采用Caspase蛋白抑制剂处理,检测Caspase-3激活情况。【结果】细胞增殖抑制实验显示藏红花素能够显著抑制耐药K562/Ima细胞的增殖,呈一定的浓度依赖关系,P<0.05。随着藏红花素浓度的提高,K562/Ima细胞凋亡率逐渐上升,表现为细胞皱缩,细胞核固缩,染色增强,出现核小体。荧光活性检测实验显示经藏红花素处理后,Caspase-3活性明显增强。采用Caspase抑制剂Z-VAD-FMK作用后,Caspase-3活性被部分抑制,K562/Ima细胞凋亡率降低。【结论】藏红花素能够抑制K562/Ima细胞的增殖,诱导其凋亡,Caspase信号通路可能参与其中。[ Objective] To investigate the proliferative inhibition and apoptotic induction effect of crocin on imatinib-resistant K562/Ima cells. [ Methods ] K562/Ima cells were seeded in the 96-well plant and treated with different concentrations of crocin for 48 h. Then CCK8 method was applied to explore the inhibition rate of crocin. The apoptosis rate of crocin on K562/Ima ceils was detected by Hoechst 33258 dying and Annexin V/PI method. Caspase activity was detected after crocin treatment, [Results] Crocin could effectively inhibit K562/Ima cells growth, with a concentration dependent pattern. The apoptotic rate of K562/Ima cells was increased as the concentration of crocin was raised. Caspase-3 was activated after crocin treatment in K562/Ima cells. Caspase inhibitor Z-VAD- FMK could effectively revise this effect. [ Conclusion ] Crocin can significantly inhibit growth and induce apoptosis of K562/Ima cells probably via Caspase pathway.

关 键 词：藏红花素 伊马替尼 慢性粒细胞性白血病 细胞凋亡 

分 类 号：R73-36[医药卫生—肿瘤]