JWA基因对食管鳞癌Ecal09细胞增殖、凋亡及侵袭的影响  

Effects of JWA gene transfection on proliferation,apoptosis and invasion of esophageal squamous cell carcinoma Ecal09 cells

作　　者：史国振[1] 谈永飞[1] 葛志军[1] 蒋国军[1] 周健[1] 

机构地区：[1]江苏大学附属宜兴人民医院心胸外科,214200

出　　处：《江苏医药》2013年第20期2383-2385,F0003,共4页Jiangsu Medical Journal

基　　金：无锡市卫生局科研基金(XM0903)

摘　　要：目的探讨JWA基因对食管鳞癌Ecal09细胞增殖、凋亡及侵袭能力的影响。方法 PCR扩增人源JWA基因序列,构建真核表达载体pcDNA3.1-JWA-GFP。通过脂质体转染将pcDNA3.1-JWA-GFP导入人食管鳞癌Ecal09细胞中(实验组);转染pcDNA3.1-CT-GFP作为对照组。测定两组细胞的细胞倍增时间、细胞凋亡率以及侵袭细胞率。结果实验组细胞倍增时间为(27.3±1.4)h,长于对照组的(23.9±1.6)h(P<0.01);实验组凋亡细胞比例也较对照组明显增加[(23.8±1.9)%vs.(8.6±0.9)%](P<0.01)。实验组的侵袭细胞率低于对照组[(11.8±1.7)%vs.(31.5±4.1)%](P<0.01)。结论 JWA基因高表达能抑制Ecal09细胞的增殖和侵袭能力,并能促进其凋亡。Objective To investigate the effects of JWA gene transfection on proliferation, apoptosis and invasion of esophageal squamous cell carcinoma Ecal09 cells. Methods Eukaryotic expression plasmid pcDNA3.1-JWA-GFP was constructed and transfected into Ecal09 cell line using Lipofectamin 2000 mediating gene transfer technigue（group A）. PcDNA3.1-CT-GFP-transfected cells were set as the controls （group B）. The detective population doubling time, apoptotic index and invasion efficiency of Ecal09 cells transfected with pcDNA3.1-JWA-GPF and pcDNA3.1-CT-GFP were examined. Results Population doubling time of group A was （27. 3±1.4） h, which was longer than （23.9±1.6） h of group B（P〈0. 01）. Apoptotic index of group A was more than that of group B [（23.8± 1.9） % vs. （8. 6 ± 0. 9） %] （P%0. 01）. The cells penetrating rate was significantly less in group A than that in group B[（11. 8±1.7）% vs. （31.5±4.1）%-] （P〈0. 01）. Conclusion Highly expressed JWA gene may suppress the proliferation and invasion ability of Ecal09 cell and promote its apoptosis.

关 键 词：JWA基因 食管鳞癌 

分 类 号：R735[医药卫生—肿瘤]