地西泮预处理对缺氧小鼠脑皮质硫化氢含量的影响  

Effect of pretreatment with diazepam on content of H2S in mice cortex underwent cerebral hypoxia

作　　者：倪云成[1,2] 汪燕[1] 张云倩[1] 马黎娜[1] 金培培[1] 许鹏程[3] 

机构地区：[1]徐州医学院麻醉学院,江苏省221004 [2]中南大学湘雅三医院 [3]徐州医学院江苏省麻醉学重点实验室,江苏省221004

出　　处：《江苏医药》2013年第20期2389-2391,共3页Jiangsu Medical Journal

摘　　要：目的观察地西泮预处理对缺氧小鼠脑皮质硫化氢(H2S)含量的影响。方法 80只昆明种小鼠随机均分为五组。其中的四组采用常压缺氧方法建立缺氧模型,分为单纯缺氧组和缺氧前30min分别腹腔注射地西泮4、8、16mg/kg三个预处理组(D4组、D8组、D16组);对照组不做任何处理。记录各组小鼠缺氧后出现张口呼吸的时间。24h后将各组小鼠断头取脑,分别测定脑含水量和皮质中H2S的含量。结果与单纯缺氧组比较,D4、D8、D16组出现张口呼吸的时间延长(P<0.05),脑含水量降低(P<0.05),D16组脑皮质H2S含量升高(P<0.05)。结论地西泮16mg/kg预处理可以促进缺氧小鼠脑皮质H2S生成,对缺氧脑损伤起保护作用。Objective To observe the effect of pretreatment with diazepam on the content of H2S in adult mice cortex underwent cerebral hypoxia. Methods Eighty Kunming adult mice were equally randomized into 5 groups of C （blank control）, H （ hypozda model control）, 134, 1）8 and D16（injected intraperitoneally with diazepam 4, 8 and 16mg/kg at 30 minutes before hypoxia, respectively）. Hypoxia model was established by atmospheric hypoxia method. The occurrence time of mouth breathing was recorded. All mice were sacrificed at 24 h after hypoxia for determining the contents of water in brain tissue and H2 S in the cortex. Results Compared with group H, the occurrence time of mouth breathing was prolonged and the content of water in brain was decreased in groups of D4,D8 and D16（P〈0. 05）, the content of H2S in the cortex was increased in group D16 （P〈0. 05）. Conclusion / Pretreatment of diazepam 16 mg/kg is helpful in protecting brain tissue from further hypoxia damage by increasing the release of H2 S.

关 键 词：地西泮 脑缺氧 硫化氢 

分 类 号：R743[医药卫生—神经病学与精神病学]