电离辐射对人肺腺癌A549细胞中STAT3靶基因表达的影响  

Effect of ionizing radiation on the expressions of STAT3 target genes in A549 cells

作　　者：高玲[2] 宋秀军 李晓 江其生 陈肖华[3] 

机构地区：[1]第二炮兵总医院全军核辐射损伤医学监测与防治研究中心 ,北京100088 [2]中国疾病预防控制中心辐射防护与核医学安全所辐射防护与核应急中国疾病预防控制中心重点实验室 [3]军事医学科学院放射与辐射医学研究所

出　　处：《中华放射医学与防护杂志》2013年第5期472-475,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（8120215,81172130,81001216）

摘　　要：目的探讨1射线照射对人肺腺癌A549细胞中促血管生成基因VEGF以及抗凋亡基因bcl-2和存活素（survivin）表达的影响及其与转录因子STAT3的关系。方法利用RT．PCR和Westernblot检测2和4Gy1射线照射后24hA549细胞中VEGF、Bel-2和Survivin表达水平以及STAT3抑制后VEGF和Survivin的表达变化。结果2和4Gy1射线照射后，VEGF和Survivin的表达均显著升高，而Bel-2的表达不受1射线照射的影响。利用抑制剂AG490阻断Y射线照射诱导的STAT3激活后，辐射诱导的VEGF和Survivin的表达升高被抑制。结论y射线照射可通过激活STAT3上调A549细胞中Survivin和VEGF的表达，Survivin蛋白的上调在一定程度上抑制了辐射诱导的肿瘤细胞凋亡，而VEGF的表达升高则有助于肿瘤血管生成。Objective To investigate the effect of ionizing radiation on the expression of transcription factor STAT3 target genes in the pulmonary adenoearcinoma cell line A549. Methods The expressions of VEGF, Bel-2 and Survivin in A549 cells with or without STAT3 inhibition were detected by RT-PCR and/or Western blot 24 h after 2 or 4 Gy of T-ray irradiation. Results After T-ray irradiation with 2 or 4 Gy, the expression of VEGF and Survivin increased significantly. However, the expression of Bcl-2 was not affected by T-ray irradiation. Furthermore, the increased expression of VEGF and Survivin induced by radiation was found to be inhibited after radiation-induced activation of STAT3 was blocked by AG490 inhibiter. Conclusions Y-ray irradiation could up-regulate the expression of Survivin and VEGF via activating STAT3. The increase of Survivin might partly inhibit the radiation-induced apoptosis of A549 cells, and the up-regulation of VEGF might contribute to the tumor angiogenesis.

关 键 词：电离辐射 STAT3 血管内皮细胞生长因子 存活素 

分 类 号：R734.2[医药卫生—肿瘤]