大鼠蛛网膜下腔出血后脑组织脂连素受体表达和血清脂连素质量浓度的变化  

Changes of expressions of AdipoR in the central nervous system and adiponectin serum concentrations after subarachnoid hemorrhage in rats

作　　者：孙鹏[1] 宋锦宁[1] 陈景宇[2] 李丹东[1] 安吉洋[1] 庞宏刚[1] 赵永林[1] 

机构地区：[1]西安交通大学医学院第一附属医院神经外科,陕西西安710061 [2]第三军医大学西南医院神经外科,重庆400038

出　　处：《西安交通大学学报（医学版）》2013年第6期735-739,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家高技术研究发展计划"863计划"基金资助项目(No.2006AA02Z4Z4);教育部新世纪优秀人才支持计划资助项目(No.NCET-05-0831);教育部高等学校博士学科点专项科研基金(No.20110201110060)~~

摘　　要：目的探讨大鼠蛛网膜下腔出血(SAH)后脑皮层和海马脂连素受体(AdipoR)表达的改变和血清中脂连素质量浓度的变化。方法将54只SD大鼠随机分为正常组(n=12)、假手术组(n=12)、SAH模型组(n=30)。采用颈内动脉穿刺法制作大鼠SAH模型。通过ELISA检测血清中脂连素的质量浓度;采用Western blot检测脂连素受体表达的变化。结果与正常组和假手术组相比,SAH后1、2、3d时血清脂连素浓度明显降低(P<0.05)。与正常组和假手术组相比,SAH模型组大鼠皮层和海马的脂连素受体1(AdipoR1)的表达明显升高(P<0.05),而脂连素受体2(AdipoR2)表达无明显变化。结论脂连素参与了SAH后早期脑损伤的病理生理过程,血清脂连素质量浓度的降低以及AdipoR1表达的增多,可能都是导致SAH后早期脑损伤的重要病理机制。Objective To investigate changes of AdipoR in the central nervous system and concentration of serum adiponectin after subarachnoid hemorrhage （SAH） in rats, Methods Fifty-four male Sprague-Dawley rats were randomly divided into three groups： normal group （n- 12）, sham-operation group（ n = 12）, and SAH model group （n =30）. An endovascular perforation model was used to induce SAH in the rats. The expression of AdipoR in the central nervous system was detected by Western blot. The concentration of serum adiponectin was detected by ELISA. Results Compared with that in normal group and sham-operation group, the concentration of serum adiponectin was decreased significantly at day 1, 2 and 3 after SAH （P〈O. 05）. Compared with that in normal group and sham-operation group, the expression of AdipoR1 in the cerebral cortex and the hippocampus was up- regulated sharply after SAH （P〈0.05）, but the expression of AdipoR2 did not change significantly （P〉 O. 05）. Conclusion Adiponectin may be involved in the pathophysiological process of early brain injury after SAH. The decreased concentration of serum adiponectin and the up-regulation of AdipoR1 expression are both important pathological mechanisms leading to early brain injury after SAH.

关 键 词：蛛网膜下腔出血 脂连素受体 脂连素 大鼠 

分 类 号：R743.35[医药卫生—神经病学与精神病学]