高迁移率族蛋白1在蛛网膜下腔出血大鼠脑组织中的表达及其意义  

Expression of HMGB1 in rats brain tissue after subarachnoid hemorrhage and its significance

作　　者：庞宏刚[1] 宋锦宁[1] 孙雷涛[2] 张明[1] 李丹东[1] 赵永林[1] 

机构地区：[1]西安交通大学医学院第一附属医院神经外科,陕西西安710061 [2]滨州医学院附属医院神经外科,山东滨州256603

出　　处：《西安交通大学学报（医学版）》2013年第6期749-753,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家高技术研究发展计划"863计划"基金资助项目(No.2006AA02Z4Z4);教育部高等学校博士学科点专项科研基金资助项目(No.20110201110060);教育部新世纪优秀人才支持计划资助项目(No.NCET-05-0831)~~

摘　　要：目的探讨蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后高迁移率族蛋白1(high mobility group protein B1,HMGB1)在血清及出血浸润脑组织内的动态表达规律。方法采用大鼠大脑前动脉穿刺法建立SAH模型,并随机分为6h、1d、3d、7d组,并设对照组。运用ELISA法测定SAH后血清中HMGB1的水平,免疫组化观察脑组织HMGB1的表达及分布,Western blot测定出血浸润区域脑组织(额底皮层)HMGB1含量。结果 SAH后血清中HMGB1水平从6h开始显著升高,并持续升高至术后7d;免疫组化结果显示,SAH后早期(6h、1d)脑组织内HMGB1表达明显减少,至晚期(3、7d)时间点其表达显著升高,并向胞质扩散;Western blot结果提示,SAH后额底皮层HMGB1呈明显早期减少、晚期升高的趋势。结论 SAH后脑组织中HMGB1水平呈早期降低、晚期升高的趋势,同时血清中HMGB1持续升高,HMGB1可能参与了SAH后脑组织的炎性损伤。Objective To explore the dynamic expression of high mobicity group protein BI（HMGB1） in rats brain tissue after subarachnoid hemorrhage （SAH）and its significance. Methods Rats SAH models were established by perforation of anterior cerebral artery, and randomly divided into 5 subgroups （control group, 6 h, 1, 3, 7 d） i ELISA was used to determine the plasm HMGB1 levels after SAH; immunohistochemical staining and western blot were used to survey the dynamic expression of HMGB1 in brain tissue after SAH. Results Plasm HMGB1 levels significantly increased after SAH, and continued to rise from 6 h to 7 d. Immunohistochemical staining indicated that HMGB1 positive cells significantly decreased within the brain tissue in early stage （6 h, 1 d） after SAH, in late stage （3, 7 d） it began to rise and translocated to cytoplasm~ Western blot results also showed the expression of HMGB1 decreased first and then increased HMGB1 expression significantly increased not only in brain role in inflammatory brain damage after SAH. in the frontal lobe cortex after SAH. Conclusion tissue but also in plasma. HMGB1 may play a important

关 键 词：蛛网膜下腔出血 高迁移率族蛋白1(HMGB1) 动态表达 炎症 脑组织 大鼠 

分 类 号：R743.35[医药卫生—神经病学与精神病学]