UVB对人视网膜色素上皮细胞GPNMB表达的影响  

Effect of UVB on glycoprotein nonmetastatic melanoma protein b in human retinal pigment epithelium cells

作　　者：司艳芳[1] 樊旭[1] 关娟[1] 周历[1] 赵娟[1] 

机构地区：[1]解放军第309医院眼科,北京100091

出　　处：《山东大学耳鼻喉眼学报》2013年第5期55-58,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的研究中波紫外(UVB)对RPE细胞GPNMB表达的影响。方法采用MTT法检测UVB对RPE细胞存活率的影响;实时定量PCR和蛋白印迹法检测UVB对RPE细胞中GPNMB表达的影响。结果 MTT实验表明,经过100μW/cm2光强的UVB辐射处理2 h后,RPE细胞会产生明显的影响,其凋亡细胞数目显著增多。RTPCR、Western blot结果表明,经UVB照射后,RPE细胞中GPNMB的表达显著上升。结论 UVB辐射可抑制RPE细胞的存活率,促进RPE细胞中GPNMB的表达。Objective To investigate the effect of ultraviolet b （UVB） on glycoprotein nonmetastatic melanoma protein b （GPNMB） in human retinal pigment epithelium （RPE） cells. Methods MTT assay was used to determine the effect of UVB on RPE cell survival rate. The effect of UVB on GPNMB in RPE cells was detected by RT-PCR and western blot. Results MTT assay showed that RPE cells were damaged by UVB irradiation at 100 μW/cm2 for 2 h and the number of apoptotic cells significantly increased. The results of RT-PCR and western blot demonstrated that the expression of GPNMB in RPE cells markedly enhanced after UVB irradiation. Conclusion UVB irradiation could sup- press the survival rate of RPE cells and promote the expression of GPNMB in RPE cells.

关 键 词：中波紫外 非转移性黑色素瘤糖蛋白B 视网膜色素上皮细胞 

分 类 号：R774.1[医药卫生—眼科]