奥沙利铂联合NK4基因对大肠癌HCT116细胞凋亡的影响  

Effects of Oxaliplatin Combined with NK4 Gene on the Function of Proliferation, Apoptosis of Colon Cancer Cell Line HCT 116

作　　者：祖丹丹[1] 李美宁[1] 张悦红[1] 张栋[1] 弓韬[1] 常冰梅[1] 

机构地区：[1]山西医科大学生物化学与分子生物学教研室,山西太原030001

出　　处：《中国医药指南》2013年第28期317-318,321,共3页Guide of China Medicine

摘　　要：目的观察奥沙利铂联合NK4基因对HCT116细胞增殖、凋亡的影响。方法使用Effectene转染试剂,将pcDNA3.1-NK4质粒转染至大肠癌HCT116细胞中,常规条件下培养24h。不同奥沙利铂药物浓度处理HCT116细胞,CCK-8法测定细胞的OD值,分析在不同奥沙利铂药物浓度下对HCT116细胞毒性的影响;检测奥沙利铂最低药物浓度联合NK4基因对HCT116细胞凋亡的影响。结果当奥沙利铂药物浓度为12.5μg/mL时,显著抑制细胞的增殖;奥沙利铂(12.5μg/mL)联合NK4基因处理组与奥沙利铂处理组比较,显著诱导HCT116细胞的凋亡,差异具有统计学意义(P<0.05)。结论与奥沙利铂组相比,奥沙利铂联合NK4基因后HCT116细胞的凋亡率显著升高。Objectives To investigate the effects of Oxaliplatin combined with NK4 gene on the function of proliferation, apoptosis of colorectal cancer cell HCT 116. Methods Effectene transfection reagent transfected pcDNA3.1 - NK4 plasmid to the colorectal cancer cells HCT 116,trained its 24 hours under the normal conditions. Deal the HCT 116 cells with Oxaliplatin different concentrations. Measure the OD value of the cells by using CCK-8 method, analysis the efects of oxaliplatin on HCT116 cell＇s toxicity under the different concentrations and the efects of the the lowest concentration of the drug and NK4 gene on the proliferation of the cells. Detect the efects of oxaliplatin/NK4 on the apoptosis of the HCT 116 cells by using V-FITC/PI double staining method combined with flow cytometry. Results When thethe oxaliplatin solution concentration is 12.5gg/mL, apoptosis appears. The statistical analysis shows that compared with oxaliplatin positive control group, the oxaliplatin/NK4 has significant differences and statistically significant（P〈0.05）. Conclusion With the oxaliplatin combined NK4 gene work on HCT 116 cells, it promote apoptosis of HCT 116 cells, thereby inhibiting the proliferation of HCT 116 cells.

关 键 词：奥沙利铂 NK4基因 HCT116细胞 凋亡 

分 类 号：R735.34[医药卫生—肿瘤]