胃癌组织中micro RNA-375基因DNA甲基化及临床意义  

DNA methylation and clinical significance of micro RNA-375 in gastric cancer

作　　者：董倩倩[1] 刘晓峰[2] 李文波[2] 尚瑞莲[2] 孙自勤[2] 魏志[2] 

机构地区：[1]辽宁医学院济南军区总医院研究生培养基地,山东济南250031 [2]济南军区总医院消化内科,山东济南250031

出　　处：《中国现代医学杂志》2013年第26期30-33,共4页China Journal of Modern Medicine

基　　金：中国博士后科学基金(No:20110490194)

摘　　要：目的探讨胃癌组织中microRNA－375基因DNA甲基化状态及其与胃癌相关临床病理因素的关系。方法收集2012年2～8月济南军区总医院20对新鲜胃癌组织和癌旁正常组织，应用甲基化特异性聚合酶链反应（MSP）技术检测组织中microRNA－375基因启动子区DNA甲基化水平，并分析其与胃癌相关临床病理因素的关系。结果microRNA－375基因启动子区甲基化阳性率在胃癌组及癌旁正常组分别为80％（16／20）和25％（5／20），差异有统计学意义（P〈O．05）。其DNA甲基化阳性率与胃癌的临床病理因素（性别、年龄、大体类型、组织学分型、TNM分期、淋巴结转移、远处转移、CEA）无显著性关系。结论胃癌组织中存在microRNA-375基因启动子区DNA甲基化．表明其可能参与了胃癌的发生和发展过程。[Objective] To investigate the DNA methylation of micro RNA-375 and its association with elinieopathologic features in gastric carcinoma. [Methods] 40 cases of gastric carcinoma and matched adjacent non-cancerous tissues was detected by DNA methylation specific polymerase chain reaction （MSP）. The relationship between hypermethylation of the gene expression level of micro RNA-375 and its clinicopathologic features of gastric carcinoma was analyzed. [Results] The positive rate of DNA methylation of micro RNA-375 gene promotor in gastric cancer and adjacent non-cancerous tissues was 80% （16/20） and 25% （5/20} respectively. There was statistically significant difference between two groups （P〈O.05）. No significant association was found between the methylation rate of micro RNA-375 and the status of age, gender, Borrmann type, TNM staging system, lymphatic metastasis and distant metastasis. [Conclusion] The hypeimethylation of micro RNA-375 gene promoter may be invovled in the initation and progression of gastric cancer.

关 键 词：胃癌 microRNA-375 DNA甲基化 MSP 

分 类 号：R735.2[医药卫生—肿瘤]