细胞周期蛋白A、D1及其mRNA在病理性瘢痕上皮和瘢痕癌中的表达及意义  

Expression and significance of cyclin A and cyclin D1 protein and its mRNA in skin pathological scar epithelium and scar cancer

作　　者：唐文台[1] 郭瑞珍[2] 王海青[2] 

机构地区：[1]遵义医学院附属医院病理科,贵州遵义563003 [2]遵义医学院珠海校区病理教研室,广东珠海519041

出　　处：《中国现代医学杂志》2013年第26期44-49,共6页China Journal of Modern Medicine

基　　金：贵州省科技攻关项目(No:2010-3080);遵义医学院自然科学类招标课题(No:F-552)

摘　　要：目的探讨Cyclin A、Cyclin D1蛋白及其mRNA在皮肤病理性瘢痕上皮、瘢痕癌组织中的的表达及意义。方法以皮肤病理性瘢痕上皮、皮肤瘢痕癌组织为研究对象,以正常皮肤表皮组织为对照。采用免疫组织化学方法(SP法)分别检测Cyclin A、Cyclin D1蛋白的表达,采用核酸分子原位杂交法检测Cyclin A mRNA、Cyclin D1 mR NA的表达,结合图像分析,分别观测正常皮肤表皮、皮肤病理性瘢痕上皮、瘢痕癌组织中检测指标的表达强度和表达水平。运用SPSS 16.0对数据进行统计学处理。结果①Cyclin A、Cyclin D1蛋白在正常皮肤表皮为阴性,在皮肤病理性瘢痕上皮呈弱阳性表达,在瘢痕癌组织中呈强阳性表达。正常皮肤表皮与皮肤病理性瘢痕上皮中的表达比较,差异无统计学意义(P>0.05),两者与瘢痕癌组织比较,差异均有统计学意义(P<0.01)。②Cyclin A mR NA、Cyclin D1 mR NA在正常皮肤表皮呈阴性表达,在病理性瘢痕上皮中呈弱阳性表达,在瘢痕癌组织中呈强阳性表达。瘢痕癌组的表达与正常皮肤表皮组及皮肤病理性瘢痕上皮组相比较,差异均有统计学意义(P<0.01);但正常皮肤表皮组与皮肤病理性瘢痕上皮组相比较,差异无统计学意义(P>0.05)。③相关分析显示,Cyclin A与Cyclin A mRNA,Cyclin D1与Cyclin D1 mRNA在皮肤瘢痕癌组织中的表达均呈正相关(P<0.01)。结论在瘢痕癌中,Cyclin A、Cyclin D1存在蛋白水平和分子水平的异常表达;其高表达可能与皮肤瘢痕癌的发生有关。[Objective] To explore the expression and signification of Cyclin A and Cyclin D1 protein and its mRNA in skin pathological scar epithelium and scar cancer tissue. [Methods] We used skin patho logical scar epithelium tissues and skin scar carcinoma tissues compared with normal skin epidermis tissues. The expressions of Cyclin A and Cyclin D1 proteins were detected by the immunohistochemical method of SP.And the expressions of Cyclin A mRNA and Cyclin D1 mRNA were detected by in situ hybridization. pictures were collected amd analyzed by computerized image-analysis, and statistical analysis was calct, lated by SPSS system. [Results] Cyclin A and Cyclin D1 showed negative expression in normal skin epidermis, weakly positive expression in skin pathological scar epithelium, strongly positive expression in scar carcinoma. The expression of levels （positive area） and intensity （mean optical density） differences were statistically significant （P〈0.01）, by compatwl with normal skin epidermis group and skin pathological scar epithelium group. But the differences of expression weren＇t statistically significant between normal skin epidermis group and skin patho logieal scar epithelium group. Cyclin A mRNA and Cyclin DI mRNA showed negative expression in normal skin epidermis, weakly positive expression in skin pathological scar epithelium, strongly positive expression in scar carcinoma. The expression of levels （positive area） and intensity （mean optical density） differences were statistically significant （P〈0.01）, by compared with normal skin epidermis group and skin pathologieal scar epithelium group. But the difterences of expression wasn＇t statistically significant between normal skin epidermis group and skin pathological scar epithelium group. Correlated analysis shows it was positive correlation that the expressions of Cyclin A and Cyclin DI in skin scar carcinoma tissues. [Conclusion] The ahnormal expression of CyclinA and Cw：lin D1 protein level and molecular level exist in skin sea

关 键 词：细胞周期蛋白 瘢痕癌 病理性瘢痕上皮 

分 类 号：R739.5[医药卫生—肿瘤]