TGF-β1对匹罗卡品癫痫大鼠海马炎性细胞因子的影响  

Effects of TGF-β1 on contents of cytokines in the hippocampus of rats after pilocarpine induced status epilepticus

作　　者：张慧敏[1] 刘益民[2] 王玉[2] 

机构地区：[1]绍兴第二医院神经内科,绍兴312000 [2]安徽医科大学第一附属医院神经内科,合肥230022

出　　处：《安徽医科大学学报》2013年第11期1316-1320,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30970997);安徽省卫生厅医学科学研究基金(编号:09B140);安徽省自然科学基金(编号:09020103008);安徽省科技攻关项目(编号:11010402168)

摘　　要：目的通过检测经鼻给予转化生长因子-β1(TGF-β1)对癫痫持续状态后大鼠海马中白细胞介素(IL)-1β、IL-6、IL-18蛋白含量的影响,探讨TGF-β1在匹罗卡品癫痫形成进程中的抗痫及神经保护机制。方法用匹罗卡品建立癫痫持续状态(SE)模型,SE后3 h,采用经鼻给予大鼠重组人TGF-β1或等量PBS溶液。各组动物于SE后24、48、72 h处死取材,应用免疫组化法确定海马区细胞因子蛋白的表达及分布特征,采用酶联免疫吸附试验(ELISA)检测海马组织IL-1β、IL-6、IL-18蛋白含量。结果 TGF-β1能有效减少癫痫大鼠海马中IL-1β和IL-6的合成与表达(P<0.05,P<0.01),对IL-18表达影响不明显。结论经鼻给予TGF-β1有效抑制了癫痫持续状态大鼠急性期海马致痫细胞因子IL-1β、IL-6蛋白的合成与表达,具有神经保护及抗癫痫形成的作用。Objective Via observing concentration changes of interleukin（IL）-1β, IL-6 and IL-18 in hippocampus of pilocarpine treated rats after intranasal delivery of transforming growth factor-β1 （ TGF-β1 ）, to explore the possible anti-epileptogenic and neuro-protective role of TGF-β1 in the epiletogenesis progression. Methods Three hours after pilocarpine induced status epilepticus, rats were treated with recombinant human TGF-β1 or the same volume of PBS by intranasal administration. Animals were executed, harvested at 24, 48, 72 h after SE individual- ly. Using the methods of the enzyme-linked immunosorbent assay （ELISA） and immunohistochemisty （ IHC）, the concentrations and distribution of IL-1β, IL-6, IL-18 were detected in hippocampus. Results TGF-β1 could reduce the expression of IL-1 β and IL-6 in the hippocampus of pilocarpine treated rats effectively （P 〈 0.05 or P 〈 0.01 ）, however, TGF-β1 did not affect the expression of IL-18 significantly （P 〉 0. 05 ）. Conclusion By the supplementation of intranasal TGF-β1, classical pro-inflammatory eytokine （IL-1β, IL-6 ） are remarkably suppressed, TGF-β1 may exert antiepileptogenic and neuropretective effects.

关 键 词：转化生长因子-Β1 白细胞介素-1Β 白细胞介素-6 白细胞介素-18 

分 类 号：R742.1[医药卫生—神经病学与精神病学]