miR-451与MIF在胃癌组织中表达的相关性研究  

Expression and clinical significance of miR-451 and MIF in gastric carcinoma

作　　者：汪伟[1] 孙晓娜[1] 张玉峰[1] 党中勤[1] 牛学恩[1] 刘晓峰[2] 

机构地区：[1]河南省中医院(河南中医学院第二附属医院)肝胆脾胃病科,郑州450000 [2]济南军区总医院消化内科,济南250003

出　　处：《安徽医科大学学报》2013年第11期1384-1386,共3页Acta Universitatis Medicinalis Anhui

基　　金：河南省科技攻关项目(编号:122102310614);郑州金水区技术研究与开发经费支持项目(编号:金科[2012]23号)

摘　　要：目的观察miR-451与巨噬细胞游走抑制因子(MIF)在胃癌组织中的表达情况,并探讨其与胃癌相关临床因素的关系。方法收集25例胃癌患者的胃癌组织和胃癌旁组织,应用RT-PCR法检测miR-451及MIF mRNA的相对表达水平,分析其相关性并探讨与胃癌相关临床因素的关系。结果 miR-451在胃癌组织中明显低表达(P<0.01),而MIF mRNA在胃癌组织中明显高表达(P<0.01),在胃癌组织中,miR-451与MIF mRNA表达呈显著负相关(P<0.01),miR-451及MIF mRNA与胃癌的分化程度、TNM分期、浸润深度及淋巴结转移相关(P<0.01)。结论 miR-451及MIF mRNA与胃癌的分化程度、TNM分期、浸润深度及淋巴结转移相关,miR-451可能通过抑制MIF负向调控了胃癌的发生、发展作用。Objective To observe the expression of miR-451 and MIF in gastric cancer tissue, and its association with clinicopathologic features in gastriccarcinoma. Methods The expression levels of miR-451 and MIF mRNA in 25 cases of gastric carcinoma and matched non tumor adjacent tissue specimens were examined using real time polymerase chain reaction （RT-PCR）. And its association with clinicopathologic features in gastric carcinoma was analyzed. Results The expression level of miR-451 was significantly lower in the tumor tissues than that in the adja- cent tissues（P 〈 0.01 ）. The expression level of MIF mRNA was significantly higher in the tumor tissues than that in the adjacent tissues（P 〈 0.01 ）. The expression of miR-451 and MIF mRNA expression was negatively correlated significantly （P 〈 0. 01 ）, and significant association was found between the status of cell differentiation of miR-451 and MIF mRNA, TNM staging system, depth of tumor invasion and tumor node metastasis stage （ P 〈 0.01 ）. Conclusion miR-451 and MIF mRNA expression is associated with the cell differentiation, TNM staging system, depth of tumor invasion and tumor node metastasis stage. UP-regulated miR-451 expression may inhibit MIF expres sion and control gastric carcinogenesis and the development process.

关 键 词：胃癌 微小RNA miR-451 巨噬细胞游走抑制因子 

分 类 号：R735.2[医药卫生—肿瘤]