辽东楤木叶总皂苷对结肠癌HT-29细胞的生长抑制作用及机制研究  

Growth inhibitory effect of total saponins from leaves of Aralia elatl Seem on human colon cancer and its related mechanism

作　　者：尹丽颖[1] 李凤金[1] 边晓燕[1] 牛雯颖[1] 敖鹏[1] 肖洪彬[1] 

机构地区：[1]黑龙江中医药大学教学实验中心,黑龙江哈尔滨150040

出　　处：《中国药理学通报》2013年第11期1545-1548,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金项目(No 30772779);国家"十二五"重大新药创制科技重大专项(No 2011ZX11102);黑龙江中医药大学优秀创新人才支持计划项目(No 051226);黑龙江中医药大学科研基金项目(No 201218)

摘　　要：目的研究辽东楤木叶总皂苷(ETS)对HT-29细胞生长的抑制作用及作用机制。方法采用MTT法检测ETS对HT-29细胞增殖的抑制作用;流式细胞仪检测ETS对HT-29细胞周期和凋亡的影响;Western blot法检测ETS对HT-29细胞PLK1蛋白表达的影响。结果 ETS对结肠癌HT-29细胞增殖有明显抑制作用,IC50值为39.62 mg·L-1。在10～40 mg·L-1剂量作用下,可剂量依赖性诱导HT-29细胞凋亡。ETS主要将细胞周期阻滞在G0/G1期,从而可剂量依赖性抑制肿瘤细胞增殖。ETS可明显抑制HT-29细胞PLK1蛋白的表达。结论 ETS体外可明显抑制HT-29细胞的生长,其抗肿瘤作用与细胞周期阻滞、细胞凋亡及抑制PLK1蛋白表达有关。Aim To investigate the growth inhibitory effects of total saponins from leaves of Aralia elatl Seem （ETS） on human colon cancer cells and explore the related mechanism. Methods The inhibitory rate of ETS on HT-29 cell proliferation was determined by MTF method. Annexin V-FITC and PI staining were used to examine cell apoptosis and cycle. The expression of PLK 1 protein was analyzed by Western blot method. Results Cell proliferation was inhibited in HT-29 cells treated with ETS, and the value of IC50 was 37.39 mg· L^-1 after 48 h. ETS could induce HT-29 cells apoptosis and arrest cell at G0/G1 phase, while decreasing the expression of PLK1 protein. Conclusions ETS can inhibit the proliferation of human colon cancer cell, and the mechanism may be related with the induction of cell apoptosis, the change of cell cycle （G0/G1 phase arrest） and the down-regulation of the expression of PLK1 protein.

关 键 词：辽东楤木 皂苷 人结肠癌HT-29细胞 细胞周期 细 胞凋亡 PLK1蛋白 

分 类 号：R-332[医药卫生] R284.1