鱿鱼墨多糖对环磷酰胺诱导的小鼠肠道黏膜损伤的保护作用及初步机制研究  

Protective effects of polysaccharides from the ink of ommastrephes bartrami on the intestinal mucosa of the mice with chemotherapy and its mechanism

作　　者：曹露[1] 左涛[1] 李学敏[1] 于卿 曹斌斌[1] 王静凤[1] 王玉明[1] 薛长湖[1] 唐庆娟[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]山东省济宁市农业委员会经管处,山东济宁272019

出　　处：《中国药理学通报》2013年第11期1558-1562,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 31071525);海洋公益性行业科研专项(No 201105029);国际科技合作项目(No2010DFA31330);泰山学者建设工程专项经费

摘　　要：目的观察北太平洋鱿鱼墨多糖对化疗小鼠肠道黏膜损伤的保护作用,并初步探讨其作用机制。方法连续给小鼠灌胃鱿鱼墨多糖28 d,d 26、27连续两天腹腔注射环磷酰胺建立化疗小鼠模型,d 29脱颈椎处死。取空肠组织进行石蜡切片,HE染色,统计绒毛高度、隐窝深度及绒毛高度与隐窝深度比值(V/C)。取肠组织匀浆进行蛋白质电泳,考马斯亮蓝染色观察特异性表达的蛋白质。取特异性表达蛋白质进行质谱分析,鉴定蛋白质种类。取肠匀浆上清检测丙二醛(MDA)和超氧化物歧化酶(SOD)活性变化。结果 (1)鱿鱼墨多糖中、高剂量组小肠绒毛高度明显高于环磷酰胺模型组(P<0.01);鱿鱼墨多糖各剂量组小肠隐窝深度均明显低于环磷酰胺模型组(P<0.01);鱿鱼墨多糖各剂量组V/C比值均明显高于环磷酰胺模型组(P<0.01)。(2)质谱结果显示,特异性表达的蛋白条带之一为谷胱甘肽巯基转移酶ω-1。(3)模型组SOD活性降低,MDA含量增高,而鱿鱼墨多糖可以提高SOD活性,降低MDA含量。结论鱿鱼墨多糖具有保护小鼠肠道黏膜的作用,预防环磷酰胺所致的消化道不良反应。上述作用与鱿鱼墨多糖能够提高小鼠肠道黏膜的抗氧化能力有关。Aim To investigate the protective effects of polysaccharides from the ink of ommastrephes bartrami on the intestinal mucosa of the mice with chemotherapy and to explore the mechanism. Methods Mice were fed continuously with squid ink polysaceharide for 28 days. Mouse model of chemotherapy was established by intraperitoneal injection of cyclophosphamide on the 26th and 27th day. All of the mice were killed on the 29th day. Ileum tissues were prepared for paraffin sections followed by HE staining, and villi length, crypt depth and V/C ratio （villus height/crypt depth） were counted. Intestinal tissues were homogenized for protein electrophoresis, and coomassie blue staining was applied to test the protein expression. Specific expressed protein was analyzed by mass spectrometry. The amount of malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） in the small intestine were also investigated. Results （1） The villi length of middle and high dose group was significantly higher than that of the cyclophosphamide model group （P 〈0.01 ）. The crypt depth of all dose group was significantly lower than that of the cyclophosphamide model group （P 〈0.01 ）. The V/C rati- os of all dose group were significantly higher than those of the cyclophosphamide model group （P 〈0.01 ）. （2） Mass spectrometry results showed that one of the specific expressed protein was glutathione S- transferases omega-1. （3）Compared with model group, the squid ink polysaccharide could improve SOD activity and reduce MDA amount. Conclusions Squid ink polysaccharide has significant protective effects on intestinal mucosa, and thus prevents cyclophosphamide induced gastrointestinal side effects, which might be related to the enhancement of antioxidant capacity in mice.

关 键 词：化疗 环磷酰胺 肠道黏膜损伤 鱿鱼墨多糖 质谱分析 抗氧化 

分 类 号：R-332[医药卫生] R282.74