LYRM1基因过表达对骨骼肌细胞活性氧的影响  

Effect of overexpression of LYRM1 gene on reactive oxygen species in skeletal muscle cells

作　　者：张敏[1] 寇春兆[2] 张春梅[1] 朱冠中[2] 季晨博[1] 郭锡熔[2] 曹新国[1] 

机构地区：[1]南京医科大学附属南京妇幼保健院儿童保健科,210004 [2]南京医科大学儿科研究所

出　　处：《中华实用儿科临床杂志》2013年第19期1467-1469,共3页Chinese Journal of Applied Clinical Pediatrics

基　　金：基金项目：国家自然科学基金（81000348）;江苏省医学创新团队项目（LJ201108）;江苏省自然科学基金（BK2011040）;南京市医学科技发展基金项目（ZKX09012）

摘　　要：目的探讨LYRM1基因过表达对骨骼肌细胞活性氧簇（ROS）生成的影响。方法培养大鼠骨骼肌细胞株（L6），分别以pcDNA3．1及LYRM1-pcDNA3．1转染IJ6，构建空载对照细胞株（空载对照组）及过表达LYRM1基因细胞株（LYRM1基因过表达组），筛选、验证细胞株LYRM1的表达。诱导M分化成熟后以H2-DCFDA探针孵育，荧光显微镜下观察ROS的荧光强度，流式细胞仪定量测定ROS的生成变化。结果LYRM1基因过表达组荧光强度明显强于空载对照组；LYRM1基因过表达组荧光值为24．8933±4．4574，空载对照组荧光值为13．1512±0．7347，2组比较差异有统计学意义（t=24．12，P=0．00）。结论LYRM1基因在骨骼肌细胞中过表达可显著增加其ROS的产生，提示LYRM1基因过表达可影响细胞线粒体功能，造成线粒体损伤。Objective To explore the effect of LYRM1 overexpression on production of reactive oxygen species （ROS） in skeletal muscle cells. Methods Rat myoblasts （ L6 ） were transfected with either an empty vector or a LYRM1 expression vector. Cells were screened and the expression of LYRM1 protein in cells was identified. L6 cells were incubated in culture solution with H2-DCFDA after they were differentiated. Then fluorescence intensity of ROS in L6 was observed by fluorescence microscope, and the content of ROS was determined by flow cytometry. Results The relative fluorescence intensity of ROS in L6 overexpressing LYRM1 was 24. 8933±4. 4574, while that in contrast cells was 13.1512±0.7347, the difference between them was significant （ t = 24.12, P = 0. 00）. Cortdusions Overexpres- sion LYRM1 can increase the production of ROS in skeletal muscle cells. LYRM1 overexpression may be influence the mitochondrial function and induce the mitochondrial damage of skeletal muscle cells.

关 键 词：肥胖 LYRM1基因 骨骼肌细胞 线粒体 活性氧簇 

分 类 号：R363[医药卫生—病理学]