偏头痛模型大鼠三叉神经脊束核神经元兴奋性增高及蛋白激酶Cε膜转位增加  

INCREASE OF NEURONAL EXCITABILITY AND PKCε MEMBRANE TRANSLOCATION IN THE SPINAL TRIGEMINAL NUCLEUS IN RATS WITH MIGRAINE

作　　者：李思颉[1] 谢娜[2] 邵国[3] 张伟[4] 胡冬梅[5] 杨明峰[5] 袁慧[5] 张颜波[5] 

机构地区：[1]首都医科大学宣武医院低氧医学研究所,北京100069 [2]山东济南市长清区人民医院,济南250300 [3]包头医学院生物医学研究中心,包头014060 [4]包头医学院第二附属医院消化科,包头014060 [5]泰山医学院附属医院神经内科,泰安271000

出　　处：《中国疼痛医学杂志》2013年第10期604-608,共5页Chinese Journal of Pain Medicine

基　　金：山东省自然科学基金(ZR2012HQ014);山东省高等学校科技计划(J10LF14)

摘　　要：目的:探讨蛋白激酶Cε(protein kinase Cε,PKCε)对偏头痛中枢敏感化大鼠三叉神经脊束核神经元兴奋性的影响。方法:实验选取200～250 g雄性SD大鼠共60只;随机均分为正常组(N组)、假手术组(C组)、偏头痛模型组(M组)、氯仿组(L组)和PKCε抑制剂H-7组(H-7组),每组大鼠12只。进行三叉神经脊束核神经元放电记录和PKCε膜转位水平检测。结果:(1)放电频率变化百分比:造模后,三叉神经脊束核细胞外放电频率增加,造模后2 h为造模前的(325.88±47.32)%;M放电频率变化百分比高于N组和C组。L组放电频率变化百分比与M相比,差异无统计学意义;H-7组放电频率变化百分比低于M和L组。(2)膜转位水平:M组、L组PKCε膜转位水平高于N组和C组;L组与M组相比,PKCε膜转位水平无明显变化;H-7组膜转位水平低于其余四组。结论:偏头痛中枢敏感化过程中,三叉神经脊束核神经元兴奋性和PKCε膜转位增加,PKCε抑制剂H-7能减少神经元兴奋性和PKCε膜转位,说明PKCε可能参与大鼠偏头痛中枢敏感化的形成。Abstract Objective： To explore the effect of protein kinase C ε（PKC ε ） on neuronal excitability in the spinal trigeminal nucleus of rats with central sensitization to migraine. Methods： Totally sixty healthy adult male SD rats, weighting from 200 - 250 g, were randomly divided into five groups： normal group （group N）, sham operation group （group C）, migraine model group （group M）, chloroform group （group L）, and H-7 group （group H-7）, with 12 rats in each group. The extracellular discharge frequency in the spinal trigeminal nucleus was recorded and PKC ε membrane translocation was tested. Results： （1） The percentage of extracellular discharge frequency change： Two hours after treatment, the percentage of discharge frequency change was （325.9 ±47.32）%. The percentage of extracellula discharge frequency change in group M was higher than that in group N and group C. There was no significant difference in the percentage of discharge frequency change between group L and group M. The percentage of discharge frequency change in group H-7 was lower than that in group M and group L. （2） PKCε membrane translocation： The PKC ε membrane translocation in groupM and group L was higher than that in group N and group C. There was no significant difference in PKC e membrane translocation between group L and group M. The PKCε membrane translocation in group H-7 was lower than the other four groups. Conclusion： During the process of central sensitization to migraine, neuronal excitability and PKC ε membrane translocation were increased. PKCε inhibitor （H-7） reduced PKC e membrane translocation and neuronal excitability, These indicated that PKC ε may play a role in central sensitization of migraine in rats.

关 键 词：蛋白激酶CΕ 偏头痛 中枢敏感化 神经元兴奋性 膜转位 

分 类 号：R747.2[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]