大黄酸水解提取物HPLC指纹图谱  

Fingerprint Chromatograms of Acid Hydrolyzed Extracts of Rheum Palmatum by HPLC

作　　者：许青霞[1] 刘彦臣[2] 刘爽[1] 赵薇[1] 牛惠芳[1] 王刚[1] 金锐[1] 李国玉[1] 

机构地区：[1]哈尔滨医科大学,黑龙江哈尔滨150086 [2]哈尔滨三乐生物工程有限公司,黑龙江哈尔滨150086

出　　处：《中医药信息》2013年第5期71-73,共3页Information on Traditional Chinese Medicine

基　　金：哈尔滨医科大学大学生创新课题(2012084)

摘　　要：目的:建立大黄酸水解提取物的HPLC指纹图谱分析方法,为其质量评价提供科学依据。方法:采用Agilent Eclipse XDB-C18ODS色谱柱(150mm×4.6mm,5μm)分离,甲醇-0.6%冰醋酸水溶液梯度洗脱;流速:0.8mL/min;检测波长:254nm。结果:7种不同产地样品的指纹图谱通过相似度计算,相似度均在80%以上。结论:该方法简便、灵敏、准确,可为该药的质量控制提供依据。Objective：To establish fingerprint chromatograms of acid hydrolyzed extracts of Rheum palmatum by HPLC.Method：Rheum palmatum was hydrolyzed by acid.Agilent Eclipse XDB-C18 ODS column（150mm×4.6mm,5μm）was used,and mobile phase was methanol-0.6％ acetic acid aqueous gradient elution with a flow rate of 0.8mL/min.The detective wavelength was 254nm.Result：The similarities of seven acid hydrolyzed extracts of Rheum palmatum from different origins were above 80％.Conclusion：The method is simple,sensitive and accurate and it can be used for the quantification control of acid hydrolyzed extracts of Rheum palmatum.

关 键 词：掌叶大黄 酸水解 高效液相色谱法 指纹图谱 

分 类 号：R284[医药卫生—中药学]