Sprouty2蛋白与人乳腺癌MCF-7细胞增殖与迁徙的关系  

Relationship between the Molecular Mechanism of Sprouty2 and Proliferation and Migration in MCF-7 Cells

作　　者：郭翔宇[1] 孙涛[1] 王妍[1] 王笑新[1] 姜翠[1] 

机构地区：[1]辽宁省肿瘤医院,辽宁沈阳110042

出　　处：《现代生物医学进展》2013年第26期5119-5122,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨Sprouty2蛋白与人乳腺癌MCF-7细胞增殖与迁徙的关系。方法:通过siRNA技术干扰MCF-7细胞sprouty2基因的表达,通过qPCR,细胞免疫荧光和western blotting检测sprouty2基因的干扰效果,MTT检测细胞增殖活力,划痕实验观察细胞迁徙能力,western blotting检测MMP-2,MMP-9和MMP-13的表达。结果:qPCR,细胞免疫荧光和western blotting检测sprouty2基因,发现sprouty2基因的下调很明显,MTT实验发现siRNA Sprouty2基因后的MCF-7细胞比对照组细胞活力明显提高,沉默组细胞的迁徙能力也明显强于对照组,且沉默sprouty2基因的MCF-7细胞,MMP-2,MMP-9和MMP-13蛋白相对对照组均上调。结论:sprouty2基因下调后,MCF-7细胞的细胞活力和迁徙能力明显提高。Objective： To explore the relationship between the molecular mechanism of Sprouty2 and proliferation and migration in MCF-7 cells. Methods： After treatment with siRNA Sprouty2 in MCF-7 cells, the Sprouty2 gene was detected by qPCR,IF and western blotting, the proliferation of MCF-7 cells were analized by methyl thiazolytetrazolium （MTT） assay,the migration of MCF-7 cells were analized by transwell assay. The expression levels of MMP-2, MMP-9 and MMP-13 in MCF-7 cells were measured by western blot. Results： After detected by qPCR, IF and western blotting, we found that the effect of knockdown of Sprouty2 was obvious, it shows that the proliferation and migration of MCF-7 cells was increased after siRNA Sprouty2, and the protein expression of MMP-2, MMP-9 and MMP-13 was upregulated. Conclusion： The proliferation and migration of MCF-7 cells was increased after siRNA Sprouty2 in MCF-7 cells.

关 键 词：Sprouty2蛋白 人乳腺MCF-7细胞 增殖 迁徙 

分 类 号：R737.9[医药卫生—肿瘤]