单纯疱疹病毒Ⅰ型感染KB细胞分析  

Analysis on the infection of herpes simplex virus type Ⅰ in KB cells

作　　者：周庆利 高金莉[2] 

机构地区：[1]濮阳市第三人民医院口腔科,河南省濮阳市457000 [2]濮阳市人民医院病理科

出　　处：《国际病毒学杂志》2013年第5期230-233,共4页International Journal of Virology

摘　　要：目的 探究KB细胞被单纯疱疹病毒Ⅰ型（herpes simplex virus,-1,HSV-1）体外感染的途径及方式.方法 取患者的疱疹液、唾液、咽喉漱洗液等标本,采用乳鼠脑内接种的方法培养分离HSV,从而获取研究所需的HSV-1,将获取的该病毒进行KB细胞感染,采用倒置显微镜鉴定病毒感染的细胞形态学改变,同时采用聚合酶链式反应技术（PCR）检测感染细胞中的HSV-1病毒的核酸,并将感染后的上清液收集起来进行乳鼠脑细胞接种,采用倒置显微镜鉴定病毒感染的细胞形态学改变.结果 在倒置显微镜下进行观察,并未发现典型细胞病变,而上清液感染后的乳鼠脑细胞则发现了典型的细胞病变,采用PCR法检测HSV-1感染KB细胞提取的DNA,可见HSV-1病毒核酸.结论 单纯疱疹病毒Ⅰ型能对KB细胞进行直接感染.Objective To explore the approach and mode of the KB cells infected by the herpes simplex virus type I （HSV-1） in vitro. Methods Collecting the herpes fluid, saliva and throat rinsing fluid from the patients. In order to gain the HSV-1 required for the research, HSV was isolated by intracere- bral injection to sucking mice. KB cells infected with the virus were identified by the inverted microscope to observe the morphological changes of the cell; the viral DNA was also detected using polymerase chain reac- tion （PCR） in the infected cells. And the supernatant was collected after infection for the intracerebral in- jection to sucking mice, and identified the morphological changes of the infected cells by the inverted micro- scope. Results The typical cytopathy was not been observed under the inverted microscope in infected KB cells, but the typical eytopathy was found in the brain cells of suckling mice intracerebral injected with the supernatant, the viral DNA was detected in the KB cells infected with HSV-1 by PCR. Conclusions The herpes simplex virus type I can directly infect the cells, and we must pay more attention to it in order to re- duce the damage of human oral epithelial cell caused by HSV-1.

关 键 词：KB细胞 单纯疱疹病毒Ⅰ型 感染 倒置显微镜 分析 

分 类 号：R781.4[医药卫生—口腔医学]