冰片与顺铂联用对Eca109(DDP^r)细胞增殖、细胞周期和凋亡的影响  

Effects of borneol combined with cisplatin on cell proliferation and cell cycle and apoptosis of human esophageal carcinoma cell line Eca109(DDP^r)

作　　者：戴翠萍[1] 张徐宁[1] 薛彩萍[1] 季宁东[1] 

机构地区：[1]淮阴卫生高等职业技术学校,淮安市消化道肿瘤重点实验室,江苏淮安223300

出　　处：《南京医科大学学报（自然科学版）》2013年第10期1388-1391,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省中医药局科技项目(LB09077);淮安市科技支撑计划项目(HAS2009002-2)

摘　　要：目的:探讨冰片与顺铂(cisplatin,DDP)联用对耐DDP的食管癌细胞Eca109(DDPr)增殖、细胞周期和凋亡的影响。方法:采用递增药物浓度持续作用诱导法建立耐DDP的食管癌细胞Eca109(DDPr),将冰片联合DDP作用于Eca109(DDPr)细胞,MTT法检测Eca109(DDPr)细胞增殖情况;流式细胞术检测细胞周期及细胞凋亡。结果:递增药物浓度持续作用诱导6个月后,细胞可以在含1μg/ml DDP的培养液中稳定生长,其对DDP的耐药指数为15.886;单用冰片浓度≤1.560μg/ml时,对Eca109(DDPr)细胞无明显抑制作用(P>0.05),但与1μg/ml DDP合用能显著促进DDP对Eca109(DDPr)细胞增殖的抑制作用(P<0.05),并促进DDP诱导Eca109(DDPr)的细胞周期改变、细胞凋亡增加,细胞周期表现为G1期细胞明显增多(P<0.05),S期细胞、G2期细胞明显减少(P<0.05),细胞的凋亡率明显增加(P<0.05)。结论:冰片促进DDP对Eca109(DDPr)细胞增殖的抑制作用,可能与改变Eca109(DDPr)细胞周期、增加细胞凋亡有关。Objective：To explore the effects of borneol combined with cisplatin （DDP） on cell proliferation and cell cycle and apoptosis of human esophageal carcinoma cell line Eca 109 （DDpr）. Methods ：The human esophageal carcinoma cell line Eca109 was exposed to continuous stepwise-increased concentration of DDP. Drug-sensitivity was measured by MTF. The cell cycle and apoptosis were determined by flow eytometry. Results：After 6 months of stepwise drug exposure, the cell could normally grow and propagate in the medium with 1μg/ml of DDP, and exhibited a resistance index of 15.886 against DDP. The cell line was named Eca109（DDpr）. MTT assay showed that borneol at the concentration of no more than 1.560 μg/ml had no obvious inhibition on the growth of Ecal09 （DDPr） cells （P 〉 0.05）,but it could significantly promote the inhibition of DDP （1 μg/ml） on the proliferation of Ecal09（DDPr） ceils （P 〈 0.05）. Compared with using DDP alone, borneol combined with DDP could change the cell cycle, significantly increase the number of cells in G1 stage （P 〈 0.05） and decrease the number of cells in S stage and G2 stage （P 〈 0.05） ,and obviously promote the apoptosis of Ecal09 （DDPr） cells （P 〈 0.05）. Conclusion：Borneol can promote the inhibition of DDP on the proliferation of Ecal09（DDPr） cells,the mechanism may be related to change cell cycle and increase apoptosis of Eca109（DDPr） cells.

关 键 词：冰片 顺铂 Eca109(DDPr) 细胞增殖 细胞周期 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤]