Toll样受体4在大鼠炎症牙髓表达的免疫组化研究  

Immunohistochemical study on dynamic expression of TLR4 in rat pulpitis

作　　者：兰卫东[1] 洪兵[1] 

机构地区：[1]南京大学附属口腔医院牙体牙髓科,南京210008

出　　处：《口腔医学》2013年第10期672-674,共3页Stomatology

摘　　要：目的研究内毒素（LPS）诱导大鼠牙髓炎模型中Toll样受体4（TLR4）的表达特点，以探讨TLR4在牙髓炎症中的可能作用，推测它在牙髓炎发生发展中的意义。方法通过对LPS诱导的大鼠磨牙牙髓炎组织切片，同时采用免疫组化方法检测大鼠牙髓炎中TLR4的表达情况。结果1d组：成牙本质细胞呈中度阳性表达，牙髓成纤维细胞为中度至强阳性表达；3～7d组：成牙本质细胞、牙髓成纤维细胞阳性表达减弱；2周组：坏死区扩大呈均质弱阳性表达，成牙本质细胞、牙髓成纤维细胞及内皮细胞减少，呈阳性，修复牙本质呈弱阳性表达；3周组：大部分坏死牙髓组织呈均质弱阳性表达。结论TLR4在大鼠牙髓炎发生、发展呈动态表达，TLR4可能参与调节牙髓炎发生、发展及修复过程。Objective To study the expression of toll like receptor-4 （TLR4） in rat pulpitis model induced by endotoxin（LPS） so as to evaluate the possible role of TLR4 in rat pulpitis and conjecture its significance in the development of pulpitis. Methods Make the slides of rat molar pulpitis tissue induced by LPS, and examine the expression of TLR4 in rat pulpitis immunohistochemically. Results 1 d group ：TLR4 was moderately positive in odontoblasts and moderately to stongly positive in the pulp fibroblasts. 3-7 d group ： the posi- tive expression reduced in odontoblasts and pulp fibroblasts. 2 weeks group ： necrosis expanded and was homogeneously weakly positive ; odontoblasts ,pulp fibroblasts and endothelial cells reduced and were positve ; reparative dentin was weakly positive. 3 weeks group ： most of the necrosis was homogeneously weakly positive. Conclusions TLR4 is dynamically expressed in the generation and development of rat pulpitis. TLR4 may take part in the regulation of generation,development and repair of pulpitis.

关 键 词：TLR4 牙髓炎 大鼠 

分 类 号：R781.31[医药卫生—口腔医学]