川芎嗪对大鼠脑缺血再灌注后occludin表达影响  

Protective effect of tetramethylpyrazine on expression of occludin after cerebral ischemia and reperfusion in rats

作　　者：李玉芳[1] 刘坤[1] 杨智航[1] 符文双[2] 巴亚萍[3] 

机构地区：[1]沈阳医学院基础医学院生理教研室,辽宁沈阳110034 [2]沈阳医学院基础医学院机能实验中心 [3]沈阳医学院11级临床本科学生

出　　处：《中国公共卫生》2013年第11期1635-1637,共3页Chinese Journal of Public Health

基　　金：沈阳市科技局计划项目(F10-205-1-21)

摘　　要：目的研究川芎嗪对大鼠脑缺血再灌注后紧密连接相关蛋白occludin表达的影响。方法采用线栓法制作大鼠脑缺血再灌注模型,应用伊文氏兰法检测血脑屏障的通透性,免疫组化法、RT-PCR法及western blot法测定缺血再灌注脑组织occludin的表达。结果 Western blot结果,缺血侧脑组织occludin蛋白表达再灌注3 h组(0.154 9±0.021 3)和72 h组(0.133 6±0.024 5)明显低于假手术组(0.334 7±0.035 7)(P<0.05),川芎嗪治疗3 h组(0.233 1±0.032 7)和72 h组(0.214 3±0.034 7)均明显高于上述同时间点再灌注组(P<0.05)。结论大鼠脑缺血再灌注后血脑屏障损伤与occludin表达下调密切相关,川芎嗪可能通过上调occludin的表达,恢复紧密连接的功能,降低缺血再灌注后血脑屏障的通透性来减轻缺血再灌注后脑水肿。Objective To study the effects of tetramethylpyrazine（TMP） on expression of occludin after brain ischemia-reperfusion（I/R）in rats.Methods The focal cerebral ischemia-reperfusion model of middle cerebral artery occlusion（MCAO） was established with intraluminal suture.The change of the permeability of blood-brain barrier（BBB） was detected using Evans blue assay.The expression of occludin was detected wih immunohischemistry assay,real-time reverse transcription PCR（RT-PCR） and western blot.Results The expression of occludin decreased significantly at the end of 3 hour and 72 hour of I/R group.The expression of occludin in TMP group was found significantly increased compared with that of the I/R group at the same time.Conclusion The down-regulation of occludin may be involved in the increase of BBB permeability induced by cerebral I/R injury;the up-regulation of occludin and recovery of function of tight junction and integrity of BBB may be one of the pathways of TMP in inhibition of cerebral edema after reperfusion.

关 键 词：川芎嗪 脑缺血再灌注 OCCLUDIN 

分 类 号：R743.3[医药卫生—神经病学与精神病学]