汤姆青霉PT95和Q1菌株产菌核培养基筛选  

Plate Culture Studies of Penicillium sp.PT95 and Q1 for Mass Production of Sclerotia

作　　者：赵文婧[1,2] 张泽清[1] 龙丹丹[1] 韩建荣[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]太原师范学院生物系,山西太原030031

出　　处：《山西大学学报（自然科学版）》2013年第4期622-627,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31070048)

摘　　要：为了确定有利于汤姆青霉PT95和Q1菌株大量产生菌核的最适培养基,选取了9种培养基进行了平板培养研究.结果表明:在质量浓度25%甘油硝酸盐琼脂培养基上,两株菌生长比较缓慢,没有渗出液和菌核的形成;Q1菌株在查氏培养基上不能形成菌核;在其他7种培养基上,两株菌都能形成菌核.PT95菌株在麦芽汁琼脂培养基上的菌核生物量最高,达到750mg/平板;在马铃薯葡萄糖琼脂培养基上的类胡萝卜素产率最高,达到23.3μg/平板.Q1菌株在麦芽汁琼脂培养基和马铃薯葡萄糖琼脂培养基上的菌核生物量最高,达到600mg/平板;在查氏酵母膏琼脂培养基上的类胡萝卜素产率最高,达到7.59μg/平板.以上结果表明PT95菌株大量产生菌核的最适培养基为麦芽汁琼脂培养基,Q1菌株大量产生菌核的最适培养基为麦芽汁琼脂培养基和马铃薯葡萄糖琼脂培养基.Nine kinds of media were used to culture the strains PT95 and Q1. The results of plate cultures showed that on the 25~ Glycerol nitrate agar medium,growth of two strains was relatively slow, and no exudate and sclerotia were found. However,Q1 strain could not form sclerotia on Czapek＇s agar medium. And on other media,two sti＇ains could form sclerotia. For PT95 strain,the highest sclerotial biomass（750 mg/plate） and carotenoid yield（23. 3 μg/plate） could be gained respectively on the malt extract agar （MEA） medium and potato dextrose agar（PDA） the highest sclerotial biomass（600 rag/plate） and medium. For Q1 strain, both MEA and PDA media gave Czapek yeast extract agar medium gave the highest carot- enoid yield（7.59 ~g/plate）. The results indicate that the optimum medium for mass production of sclerotia was MEA medium for strain PT95 and MEA and PDA media for strain Ql,respectively.

关 键 词：类胡萝卜素 培养基 汤姆青霉 平板培养 菌核 

分 类 号：Q934[生物学—微生物学]