透骨草提取物对人卵巢SKOV-3细胞PI3k/Akt/mTOR信号通路的影响  

The Influence of Phryma Leptostachya L. var. asiatica Hara Extract on Expressions of PI3k /Akt /mTOR Signal Pathway in SKOV-3 Cells

作　　者：刘立新 曾常茜[2] 陈群[2] 

机构地区：[1]吉林省汪清县东城卫生院检验科,吉林汪清133207 [2]大连大学医学院,生物有机化学重点实验室,辽宁大连116622

出　　处：《中医药学报》2013年第5期57-59,共3页Acta Chinese Medicine and Pharmacology

基　　金：2010年大连市科学技术基金计划项目(2010E13SF144)

摘　　要：目的:研究透骨草提取物对人卵巢SKOV-3细胞PI3k/Akt/mTOR蛋白表达的影响,探讨透骨草提取物对SKOV-3细胞作用的分子机制。方法:免疫组化法检测透骨草提取物对SKOV-3细胞PI3k/Akt/mTOR蛋白表达的影响。结果:37.8μg/mL透骨草提取物作用于SKOV-3细胞24h后,SKOV-3细胞PI3k/Akt/mTOR蛋白表达明显低于与对照组(P<0.05),表明透骨草提取物可下调SKOV-3细胞PI3k/Akt/mTOR蛋白的表达。结论:透骨草提取物可能通过PI3k/Akt/mTOR途径抑制SKOV-3细胞增殖。Objective：To study the influence of Phryma Leptostachya L.var.asiatica Hara extract on expressions of PI3k,Akt and mTOR proteins in SKOV-3 cells and investigate the related molecular mechanism.Methods：The expressions of PI3k,Akt and mTOR proteins in SKOV-3 cells were examined with immunohistochemistry technique.Results：The expressions of PI3k,Akt and mTOR proteins in SKOV-3 cells treated with 37.8μg/mL Phryma Leptostachya L.var.asiatica Hara extract for 24h decreased compared with the control group respectively （P ＜ 0.05）,indicating Phryma Leptostachya L.var.asiatica Hara Extract could downregulate expressions of PI3k,Akt and mTOR proteins in SKOV-3 cells.Conclusion：Phryma Leptostachya L.var.asiatica Hara Extract could inhibit the proliferation of SKOV-3 cells by PI3k/Akt/mTOR signal pathway.

关 键 词：透骨草提取物 SKOV-3细胞 PI3K AKT MTOR 

分 类 号：R285.5[医药卫生—中药学]