应用rDNA ITS区测序评估API20C AUX及CHROMagar对酵母菌的鉴定  

Evaluation of Identification Method of API 20C AUX and CHROMagar with rDNA ITS Sequential Analysis for Yeast

作　　者：陈中举[1] 孙自镛[1] 李丽[1] 朱旭慧[1] 张蓓[1] 田磊[1] 简翠[1] 汪玥[1] 闫少珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院检验科,武汉430030

出　　处：《现代检验医学杂志》2013年第5期68-71,共4页Journal of Modern Laboratory Medicine

摘　　要：目的 使用真菌内转录间隔区（ITS ）测序分析鉴定酵母菌的方法评估API 20C AUX和CHROMagar两种鉴定方法,为临床实验室选择快速、准确鉴定酵母菌的方法提供依据.方法 收集同济医院2010～2012年临床分离的172株酵母菌,以ITS区测序分析鉴定方法为标准,对API 20C AUX及CHROMagar 24 h,48 h显色鉴定方法进行评估,计算其鉴定符合率.结果 与测序分析比较,API20C AUX鉴定对白色念珠菌、热带念珠菌、光滑念珠菌、克柔念珠菌的鉴定符合率均为100%,对新型隐球菌及近平滑念珠菌的鉴定符合率分别为95%和97.6%;CHROMagar对白色念珠菌和克柔念珠菌24 h和48 h鉴定符合率均为100%,对热带念珠菌24 h和48 h鉴定符合率分别为85.3%和94.1%,对光滑念珠菌24 h和48 h鉴定符合率分别为70%和83.3%,对新型隐球菌和近平滑念珠菌不能进行鉴定.结论 API 20C AUX鉴定系统可以作为临床实验室常规鉴定的手段,对于CHROMagar鉴定培养基只能用于实验室对酵母菌的初步筛查,对白色念珠菌、克柔念珠菌、光滑念珠菌和热带念珠菌的鉴定效果较好,对其他念珠菌及隐球菌的鉴定还需其他方法进行补充.Objective This study has made use of fungus internal transcribed spacer,ITS, sequencing and analysis to evaluate whether or not API 20C AUX and CHROMagar could be available in identification of clinical deeper infected yeasts,provi-ding database of methodology for identification of the fungal pathogens in diagnostic microbiological laboratory. Methods A total of 172 strains of yeasts were isolated from Tongji Hospital during 2010-2012 and DNA was extracted for ITS region sequencing and analysis. Meanwhile API 20C AUX and CHROMagar 24 h,48 h color identification method was conducted. Based on the ITS region sequencing and analysis,API 20C AUX and CHROMagar were evaluated and coincidence rates of i- dentification were calculated. Results Evaluation of API 20C AUX and CHROMagar in identification of deeper yeasts ： Com-pared with ITS sequencing data analysis, the coincidence rates of API20C AUX for identification of＇Candida albicans, Can-dida tropicalis , Candida glabrata , Candida krusei were 100%, Cryptococcus neo f ormans and Candida para psilosis were 95% and 97.6 % respectively. Identification accuracy of CHROMagar of Candida albicans and Candida krusei at 24h and 48h were 100%, Candida tropicalis at 24h and 48h were 85.3 % and 94. 1% respectively. Candida glabrata at 24h and 48h were 70% and 83.3%. Cryptococcus neoformans and Candida parapsilosis was not identified by CHROMagar. Conehlsion API 20C AUX identification system can be used as a routine clinical laboratory identification means,but CHROMagar cul-ture medium can only be used for laboratory preliminary screening of deeper yeasts with exception of Candida albicans, Candida krusei and Candida glabrata and Candida tropicalis that can be identified well by CHROMagar. Other Candida and Cryptocaccus may need to be supplemented with other methods.

关 键 词：酵母菌 内转录间隔区 API 20C AUX CHROMAGAR 

分 类 号：R379[医药卫生—病原生物学] Q786[医药卫生—基础医学]