ET-1与扩张皮瓣微循环关系的实验研究  

Experimental Study on the Relationship of Endothelin-1 and Expanded Flap Microcirculation

作　　者：玉光哲[1] 夏昊晨[1] 安宇[1] 王凯夫[1] 杨硕[1] 司振兴[1] 金英玉[1] 

机构地区：[1]哈尔滨医科大学附属第一医院急诊外科,哈尔滨150001

出　　处：《现代检验医学杂志》2013年第5期105-107,共3页Journal of Modern Laboratory Medicine

基　　金：黑龙江省中医药科研项目（编号：ZHYl2-Z181）.

摘　　要：目的探讨扩张皮瓣转移术后皮瓣微循环与内皮素-1（ET-1）的关系。方法用7只小型猪，模拟成扩张皮肤转移术后淤血皮瓣42个，分成7组，即术前、术后1，3，6，9，12和24h，用激光多谱勒血流测定仪测距蒂部8cm处的背部皮肤微循环血量和放免法测定远端（距蒂部8cm），近端（距蒂部1cm）皮瓣ET-1的含量，并在24h内连续观察皮瓣颜色。结果扩张皮瓣近端ET-1含量1h与术前相比有增高趋势，3h增高显著（t=2．32，P〈0．05），6h达到峰值（t=3．18，P〈0．01），随后开始下降。扩张皮瓣远端ET-1含量则无明显变化。远端淤血部微循环与近端ET-1有显著负相关（R=-0．755，P〈0．01）。结论扩张皮瓣转移术后皮瓣近端（蒂部）ET-1含量对远端微循环起重要调节作用。Objeetive To evaluate the relationship of endothelin（ET） and flap microcirculation. Methods Seven mini pigs were employed to establish expansion models and 42 expanded flap made were divided into 7 groups（before surgery, 1,3,6, 9,12,24 h after surgery）. After different hours,distal microcirculation were detected by dopler and both distal and base ET level were measured by radio-immunity. Expanded flap colour were observed. Results Compared to ET-1 level before sur-gery, ET-1 levle of 1 h after surgery group increased, 3 h after surgery group had significant difference （t=2.32,. 05）, 3 h after surgery group got a peak （t= 3.18,0.01）, and sbusequently decreased. Distal microcirculation had statistically positive correlation with base skin ET level （R=-0. 755 ,P〈0.01）. Conclusion Base ET had important effect on distal blood flow after expansion.

关 键 词：扩张皮瓣 内皮素-1 微循环 

分 类 号：R-332[医药卫生]