甘薯对潮霉素及除草剂的敏感性研究  

Sensibility of Ipomoea batata to Hygromycin and Basta

作　　者：李倩[1] 张聪[2] 吴洁[2] 蒲志刚[2] 王大一[3] 阎文昭[2] 邓科君[1] 张勇[1] 郑雪莲[1] 

机构地区：[1]电子科技大学生命科学与技术学院,四川成都610054 [2]四川省农业科学院生物技术核技术研究所,四川成都610066 [3]四川省农业科学院作物研究所,四川成都610066

出　　处：《西南农业学报》2013年第5期1870-1872,共3页Southwest China Journal of Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)(2006AA100107);"十一五"国家科技支撑计划(2007BAD78B00);国家自然科学基金(31000735);中央高校基本科研业务费(ZYGX2012J109);四川省农作物育种攻关项目甘薯育种子课题(2011-2015);四川省基因工程育种项目(2011JYGC01-005)

摘　　要：为了研究不同品种甘薯在潮霉素以及basta选择下的耐受性,为甘薯的遗传转化提供依据,本试验选取了四川省常用甘薯栽培品种（材料）,川薯164与西成薯007,以叶片为外植体,分别接种于含有6-BA、NAA及不同浓度梯度的潮霉素及basta的培养基上,统计分析得出不同基因型甘薯的最适抗性压.结果表明,川薯164与西成薯007叶片抗hyg的最适选择压分别为20～30,15～20 mg/L,川薯164与西成薯007叶片抗basta的最适选择压均为1.0 mg/L.In order to study the tolerance of Ipomoea batata to hygromycin and basta and to provide a basis for genetic transformation of Ipomoea batata,Sichuan common cultivars （materials）,Chuanshu164 and Xichengshu 007 were selected,of which blades were used as explants to respectively be inoculated to the medium containing 6-BA,NAA and different concentration gradient of hygromycin and basta,and the optimal resistance pressures of gain different genotypes of Ipomoea batata were analyzed.The results showed that the ideal selecting pressure of hygromycin was 20-30 mg/L in Chuanshu 164 blade and 15-20 mg/L in Xicheng 007 blade,and the ideal selecting pressure of basta was 1.0 mg/L in both Chuanshu 164 and Xicheng 007 blade.

关 键 词：甘薯 敏感性 潮霉素 Basta 愈伤组织 褐变 

分 类 号：S531[农业科学—作物学]