hMAM mRNA在乳腺癌组织中的表达  

The Expression of Mammaglobin Gene in Breast Cancer

作　　者：刘宁[1] 张伟[2] 邵永孚[2] 王洪平[2] 金玉生[2] 

机构地区：[1]天津医科大学肿瘤医院腹部外科,300060 [2]中国医学科学院中国协和医科大学肿瘤医院,100021

出　　处：《实用癌症杂志》2000年第6期580-582,共3页The Practical Journal of Cancer

摘　　要：目的　探讨乳腺组织特异性基因hMAMmRNA作为检测乳腺癌微小转移指标的可能性。方法　采用RT PCR方法 ,检测hMAMmRNA在乳腺癌组织和相应癌旁正常乳腺组织 44例及胃癌、大肠癌、食管癌、肺癌、卵巢癌组织各 5例中的表达。分析hMAMmRNA表达与乳腺癌临床病理特点之间的关系。实验结果进行统计学处理。结果　 44例乳腺癌组织和相应癌旁组织中均表达hMAMmRNA ,其中有 5 6 .82 % (2 5 /4 4 )癌组织相对正常组织呈高表达 (P >0 .0 5 ) ;而胃癌、大肠癌、食管癌、肺癌、卵巢癌均无表达。hMAM在乳腺癌组织和其它癌组织中的表达有显著性差异 (P <0 .0 1)。hMAM表达与乳腺癌病理类型、临床分期、ER、PR状态无相关性 (P >0 .0 5 )。结论　hMAMmRNA作为乳腺癌组织特异性标志物 ,可能成为检测乳腺癌微小转移的指标。Objective To determine the possibility of detecting hMAM mRNA expression as a marker of micrometastasis in breast cancer.Methods Total RNA was extracted from a breast carcinoma cell line (SKBR3),44 primary breast carcinomas and corresponding normal tissues,and 25 other carcinomas(including gastric carcinoma,esophageal carcinoma,lung carcinoma,ovary carcinoma and carcinoma of the large intestine) and was analyzed for hMAM mRNA expression by RT-PCR method.The relationship between clinicopathological charactristics of the patients and the results of RT-PCR was analyzed statistically.Results hMAM mRNA expression was detectble in SKBR3 cells and in all 44 breast carcinomas but was not in other cancers.In the analysis,hMAM mRNA levels were increased in 56.82%(25/44) of corresponding normal breast tissue(P>0.05).And there was no expression in other cancers.There is obviously different between breast cancer and other cancers in the expression of hMAM gene(P<0.01).There was no relationship between the expression of hMAM gene and stage,statuses of ER and PR of patients(P>0.05).Conclusion hMAM mRNA is a special marker of the breast tissue and that may be a marker of micrometastasis of breast cancer.

关 键 词：乳腺癌 乳腺球蛋白 HMAM MRNA 表达 

分 类 号：R737.902[医药卫生—肿瘤]