膜表面核仁素参与表皮生长因子受体信号启动的研究  

Research on The Membrane Surface Nucleolin Participated The Activation of Epidermal Growth Factor Receptor Signaling

作　　者：杨香山[1] 范开席[2] 李成军[3] 李爱萍[4] 张晶[1] 冯曼[1] 

机构地区：[1]山东省医学科学院附属医院病理科,山东济南250031 [2]山东省医学科学院附属医院中心实验室,山东济南250031 [3]山东省医学科学院附属医院普外科,山东济南250031 [4]山东省医学科学院附属医院内科,山东济南250031

出　　处：《中国普外基础与临床杂志》2013年第10期1117-1121,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨膜表面核仁素(nucleiolin,NCL)在表皮生长因子受体(epidermal growth factor receptor,EGFR)信号启动中的作用。方法采用免疫组化法检测NCL及EGFR在甲状腺乳头状癌组织中的表达;Western blot法检测甲状腺乳头状癌细胞TPC-1中磷酸化EGFR(phosphorylation EGFR,p-EGFR)的表达水平;Transwell小室试验检测TPC-1细胞的迁移能力。结果免疫组化染色结果显示,甲状腺乳头状癌组织中NCL及EGFR的表达阳性率分别为100%(56/56)和80.4%(45/56);NCL及EGFR的表达与淋巴结转移有关(P<0.05),且NCL的表达与EGFR的表达呈正相关关系(r=0.635,P<0.01)。Western blot法检测结果显示,拮抗甲状腺乳头状癌TPC-1细胞的NCL或EGFR后,其p-EGFR表达水平均明显下调(P<0.01)。Transwell小室试验发现,拮抗NCL和EGFR后,可明显减少TPC-1细胞的穿膜细胞数(P<0.01)。结论膜表面NCL可能是EGFR受体信号启动的必要成分,可能通过EGFR参与肿瘤的生长与转移。以NCL为靶点将开拓肿瘤新的治疗领域。Objective To explore the effect of membrane surface nucleolin （NCL） on activation of epidermal growth factor receptor （EGFR） signaling. Methods Immunohistochemistry was used to identify the expressions of membrane surface NCL or EGFR in pallilary thyroid carcinoma （PTC） tissues. The level ofphosphorlated EGFR in TPC-1 cells was observed by Western blot. TPC-1 cells invasion capacity was detected by Transwell assay. Results The posi- tive expression rates of membrane surface NCL and EGFR in PTC tissues were 100% （56/56） and 80.4% （45/56） respe- ctively, while the expressions of NCL and EGFR were related with lymph node metastasis （P〈 0. 05）. There was posi- tive correlation between the expressions of NCL and EGFR （r=-0. 635, P〈 0. 01）. Western blot showed that anti-NCL or anti-EGFR of TPC-1 cells could inhibit the expression of phosphorlation EGFR （P〈 0. 01）. Transwell assay showed the number of membrane-invading cells were reduced significantly in anti-NCL group anti-EGFR group （P〈 0.01）. Conclusions Membrane surface NCL may be a kind of indispensable component in activation of EGFR signaling, by which EGFR can participate in growth and invasion of tumors. NCL can be used as a target for developing a new field of tumor treatment.

关 键 词：膜表面核仁素 表皮生长因子受体 信号启动 

分 类 号：R73-3[医药卫生—肿瘤]