介入性热灌注联合瘤体注射热碘油对兔VX2肝癌模型的疗效研究  

The therapeutic effect of interventional heated-Lipiodol perfusion combined with heated-Lipiodol injection into tumor for the treatment of VX2 liver cancer in experimental rabbits

作　　者：何伟华[1] 独建库[1] 张明德[1] 邹洋[1] 马小桔[1] 

机构地区：[1]河南洛阳解放军第150医院介入中心,471000

出　　处：《介入放射学杂志》2013年第11期940-942,共3页Journal of Interventional Radiology

基　　金：军队十二五课题资助项目(08z006)

摘　　要：目的观察介入性灌注热碘油联合瘤体注射热碘油对兔VX2肝癌模型的疗效及安全性。方法将VX2肝癌细胞接种于30只新西兰白兔肝内,制备肝VX2模型。将动物随机分为实验组和对照组,每组15只。实验组肝动脉灌注联合经皮穿刺瘤内注射60℃热碘油3 ml,对照组经肝动脉灌注常温25℃碘油3 ml。用B超观察栓塞前后瘤体大小,采血查血清丙氨酸转氨酶(ALT)水平。结果实验组肿瘤体积由(1 565±315)mm3缩小至(1 054±463)mm3;对照组肿瘤体积由(1 568±323)mm3缩小至(1 275±472)mm3,组间差异有统计学意义(P<0.05)。实验组血清ALT从(736±212)u/L增至(816±247)u/L,对照组从(745±215)u/L增至(796±236)u/L,组间差异无统计学意义(P>0.05)。结论介入性灌注热碘油联合瘤体注射热碘油对兔VX2肝癌模型有更强的抑制作用。Objective To explore the therapeutic effect and clinical safety of interventional heated- Lipiodol perfusion combined with heated-Lipiodol injection into tumor for the treatment of VX2 liver cancer in experimental rabbits. Methods VX2 liver cancer cells were inoculated into the livers of 30 New Zealand white rabbits to prepare the models of liver VX2. The animals were randomly and equally divided into study group （n = 15 ） and control group （n = 15）. Interventional hepatic arterial infusion of heated-Lipiodol perfusion （60℃, 2 ml） was performed in the rabbits of the study group, which was followed by heated-Lipiodol （60℃, 1 ml） injection into the tumor three days later. For the rabbits of the control group, only transhepatie arterial infusion of 3 ml room-temperature （25℃） Lipiodol was employed. Before and after the procedure, the tumor sizes were measured by color Doppler ultrasonography and the serum ALT levels were determined. The results were analyzed and compared between the two groups. Results The tumor volume of the study group decreased from preoperative （1 565 ± 315）mm3 to postoperative （1 054 ± 463）mm3, while the tumor volume of the control group decreased from preoperative （1 568 ± 323）mm3 to postoperative （1 275 ± 472）mm3, the difference between the two groups was statistical significant （P 〈 0.05）. The serum ALT level was increased from preoperative （736 ± 212）u/L to postoperative （816 ± 247）u/L in the study group, while in control group it was increased from preoperative （745 ± 215）u/L to postoperative （796 ± 236）u/L, and statistically significant difference existed between the two groups （P 〉 0.05）. Conclusion Interventional heated-Lipiodol infusion combined with heated-Lipiodol injection into the tumor can more strongly inhibit the VX2 liver cancer than conventional transhepatie arterial infusion of 3 ml room-temperature （25℃） Lipiodol does in experimental rabbit models.

关 键 词：肝肿瘤 热碘油 动物实验 

分 类 号：R735.7[医药卫生—肿瘤]