常压缺氧致大鼠脑损伤无创检测中脑电图近似熵的应用  

Application of approximate entropy in non-invasive detection of cerebral normobaric hypoxia damage

作　　者：徐新萍[1] 王德文[1] 彭瑞云[1] 李英俊[1] 左红艳[1] 王少霞[1] 李杨[1] 王水明[1] 孙成峰[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国体视学与图像分析》2013年第3期295-301,共7页Chinese Journal of Stereology and Image Analysis

基　　金：国家709项目(2006FK130006)

摘　　要：目的基于近似熵对常压10%缺氧后大鼠脑电图(EEG)进行分析,探讨应用近似熵衡量缺氧对脑损伤程度的可行性。方法 Wistar雄性大鼠70只,随机分为对照组和10%的缺氧组;采用多导生理仪分别于缺氧前及持续缺氧6 h、1 d、3 d、7 d及14 d时,对大鼠EEG及近似熵进行检测;采用HE染色观察大鼠大脑皮层组织结构变化;采用图像分析方法对变性细胞进行定量分析。结果 (1)与对照组和缺氧前比较,1 d开始降低,于7 d显著低于对照组和缺氧前水平(P<0.05),缺氧14 d后近似熵基本恢复至正常水平;(2)缺氧6 h后,大脑皮层见少量神经元发生缺氧性改变,缺氧7 d内进行性加重,病变范围逐渐增大,14 d明显恢复。结论 (1)10%缺氧可引起大鼠脑电生理功能损伤,且与缺氧时间相关;(2)近似熵可客观反映缺氧对脑功能的损伤,可望成为一个新的检测缺氧后脑损伤的敏感指标。Objective 10% O2 （ApEn） randomly We analyze rat electroencephalogram （EEG） based on ApEn after normobarie hypoxia-induced cerebral injury, to explore the feasibility of in the evaluation of bypoxia-induced brain damage. Methods divided into control group and hypoxia group （ 10% O2）. EEG applying approximate entropy Seventy male Wistar rats were and ApEn of the rats were recorded with a multi-channel physiological system before hypoxia, 6 h, 1 d, 3 d, 7 d and 14 d after hypoxia, respectively. Histopathology using HE staining was used to observe the structural changes of cerebral tissue, and then the degenerated cells were quantified by image analysis. Results （1） Compared with the control group and experimental group before hypoxia,ApEn was decreased at 1 d and 7 d, especially showed a significant reduction 7 d after hypoxia, then it recovered to the normal leval at 14 d posthypoxia ; （ 2 ） Hypoxic 6 h after hypoxia, w path hich ologieal changes occurred in a small number of neurons in the cerebral cortex at were aggravated gradually within 7 d, and the area of pathological changes extended. Above-mentioned changes recovered obviously 14 d after hypoxia. Conclusions （1） 10% O2 hypoxia can induce electrophysiological alterations in the brain, and the changes are related to the time of hypoxia; （2） ApEn can objectively reflect the hypoxia-induced functional damage of the brain, and monitoring and prediction of ApEn may become a new sensitive indicator in the evaluation of brain damage caused by hypoxia.

关 键 词：近似熵 缺氧 脑电图 图像分析 大鼠 

分 类 号：R363[医药卫生—病理学]