阿霉素联合他莫昔芬对人子宫内膜癌裸鼠移植瘤的抑制作用  

Experimental study on the inhibitory effect of adriamycin combined with tamoxifen on transplanted tumor in vivo human endometrial carcinoma in nude mice

作　　者：贾玖丽[1,2] 马晓欣[1] 柯孝瑜[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]平顶山第一人民医院妇产科,河南平顶山467000

出　　处：《现代肿瘤医学》2013年第11期2423-2427,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(No.81272874);辽宁省科学技术厅资助项目(No.2011404013-8);沈阳市科学技术局资助项目(No.F12-227-1-62)

摘　　要：目的:分别研究阿霉素、他莫昔芬及两者联合用药对人子宫内膜癌裸鼠移植瘤的生长抑制作用及相应机制;方法:建立裸鼠活体二次移植肿瘤的动物模型,并随机分为4组,每组5只。分别为阿霉素用药组、他莫昔芬用药组、阿霉素他莫昔芬联合用药组及对照组;用药处理裸鼠移植瘤模型3周。对照组予以注射生理盐水3周。计算各组移植瘤体积、抑瘤率及裸鼠体重情况,肿瘤组织标本进行免疫组化检测Ki67蛋白的表达,并用TUNEL法检测细胞凋亡情况。结果:联合用药组与单独用药组及对照组的瘤体积比较差异均有统计学意义(P<0.05);用药前各组裸鼠体重差异无统计学意义(P>0.05),用药后对照组与单独用药组及联合用药组的体重比较差异均有统计学意义(P<0.05);联合用药组与单独用药组及对照组的抑瘤率比较差异均有统计学意义(P<0.05);用药组的Ki67表达阳性率明显低于对照组,联合用药组Ki67表达最低;TUNEL法检测联合用药组肿瘤细胞凋亡率为(32.33±3.68)%,明显高于阿霉素组(17.40±2.58)%、他莫昔芬(15.40±6.05)%和对照组(3.50±1.50)%,差异有统计学意义(P<0.05)。结论:阿霉素、他莫昔芬均可抑制人子宫内膜癌裸鼠移植瘤的生长,两者联合应用较两药单独应用对人子宫内膜癌裸鼠移植瘤的抑制作用更为显著。Objective：To study inhibitory effect and mechanism of tamoxifen and adriamycin alone,and combined two drugs on human endometrial cancer xenografts in nude mice.Methods：To establish nude mouse animal model in vivo two transplant tumor.The nude mice were randomly divided into 4 groups,with 5 rats in each group,adriamycin group,tamoxifen group,adriamycin tamoxifen combined treatment group and control group.The nude mouse model of drug treatment for 3 weeks,the control group was given injection of normal saline for 3 weeks.Calculated the tumor volume,tumor inhibition rate and body weight of nude mice.The expression of Ki67 protein of tumor tissue specimens was tested by immunohistochemical detection,and TUNEL method was used to detect cell apoptosis.Results：For combined group,monotherapy group and control group,there were significant differences in tumor volume （P ＜ 0.05） ; no statistical significance was observed before and after the treatment for nude mice weight （P ＞ 0.05）,but for the control group and the treatment group,the weight,was significantly different （P ＜ 0.05）,the tumor inhibition rate was different （P ＜ 0.05）.The positive rate of Ki67 in treatment group was significantly lower than that in control group,combined treatment group Ki67 was the lowest.TUNEL method was used to detect the apoptosis of tumor cells combined with medicine group was （3 2.3 3 ± 3.6 8） ％,significantly higher than single medication group ADM group （17.40 ± 2.58） ％,tamoxifen （15.40 ± 6.05） ％ and the control group （3.50 ± 1.50） ％,the difference was statistically significant （P ＜ 0.05）.Conclusion：Adriamycin,tamoxifen can inhibit the human endometrial cancer xenografts in nude mice,the combination of the two drugs was more effective than single use on human endometrial cancer xenografts in nude mice.

关 键 词：BALB/C裸鼠 子宫内膜癌 阿霉素(ADM) 他莫昔芬(TAM) Ki67 TUNEL 

分 类 号：R73-361[医药卫生—肿瘤] R737.33[医药卫生—临床医学]