Foxm1对非小细胞肺癌细胞迁移、浸润的影响  

The effect of Foxm1 on the migration and invasion of non small cell lung cancer (NSCLC) cells

作　　者：孔飞飞[1] 袁海花[1] 王炯轶[1] 赵美[1] 郭跃辉[1] 时婧[1] 公小迪[1] 王竞[1] 姜斌[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院肿瘤科,上海201900

出　　处：《现代肿瘤医学》2013年第11期2427-2431,共5页Journal of Modern Oncology

基　　金：上海市教委优秀青年教师基金(编号:zzjdyx12111);上海交通大学医学院科技基金项目(新华集团基金项目;编号:11XJ22014);上海交通大学医学院附属第三人民医院院基金(编号:syz2011-05)

摘　　要：目的:探讨Foxm1在非小细胞肺癌细胞EMT过程中的作用进而研究其对非小细胞肺癌细胞迁移和浸润能力的影响。方法:采用Western blot和RT-PCR技术检测不同非小细胞肺癌细胞系中Foxm1和EMT相关因子的表达水平。小RNA转染技术干扰H1299细胞中Foxm1的表达。Transwell试验观察Foxm1对非小细胞肺癌细胞迁移浸润能力的影响。结果:Foxm1在四种非小细胞肺癌细胞中均有表达,且H1299表达量相对较高,H1650表达量相对较低。分别以上述两种细胞为试验对象,结果显示Foxm1的表达与EMT相关分子E-cadherin、Vimentin的表达呈明显相关性。CCK-8结果显示:Foxm1抑制剂Thiostrepton处理72h后对H1299、H1650的IC50值分别为1.21μmol/L和3.08μmol/L。Thiostrepton处理和干扰后,Foxm1和Vimentin的表达量明显下降。Transwell小室迁移试验24h后,空白组、阴性对照组和干扰组穿膜细胞数分别为42.6±6.9,36.3±4.2,4.6±1.1;侵袭试验24h后,空白组、阴性对照组和干扰组穿膜细胞数分别为12.3±3.4,10.0±1.4,3.1±2.6。结论:Foxm1在非小细胞肺癌细胞中可能通过调节EMT进程来促进肿瘤细胞的迁移和浸润能力。Objective：To observe the role of Foxm1 in the process of EMT to analyse its effect on the migration and invasion of NSCLC cells.Methods：The basic expression level of Foxm1 and EMT related factors were detected by Western blot and RT-PCR.We used the transfection technology to knockdown the expression of Foxm1 in H1299 cells.The inhibition rate of cell proliferation was examined by CCK-8.The capabilities of migration and invasion was measured by transwell assay.Results：Foxm1 can be detected in four lung cancer cell lines enrolled in our research and the expression level in H1299 cells was obviously higher than H1650 cells.And then,we selected H1299 and H1650 cells into our following studies.There was an obvious relation between the expression of Foxm1 and EMT related factors E-cadherin and Vimentin.The IC50 of thiostrepton,a kind of Foxm1 inhibitor,were 1.21 μmol/L and 3.08 μmol/L in H1299 cells and H1650 cells,respectively.After the treatment of thiostrepton and the transfection of Foxm1,the expression of Foxm1 and Vimentin were decreased obviously.At 24 hours after transwell migration and invasion assay,the cell count of each group as control,negative control and siFoxm1 was 42.6 ± 6.9,36.3 ± 4.2,4.6 ±1.1 and 12.3 ± 3.4,10.0 ± 1.4,3.1 ± 2.6,respectively.Conclusion：Foxm1 can improve the migration and invasion capabilities of NSCLC cells through the regulation of EMT process.

关 键 词：FOXM1 上皮间质转化 E钙黏素 波形蛋白 

分 类 号：R734.2[医药卫生—肿瘤]