细胞DNA倍体定量分析在宫颈癌与癌前病变中的应用探讨  

Application of DNA cytometry for identification of cervical squamous cell carcinoma

作　　者：张玉[1] 景彩萍[1] 霍媛媛[1] 李根[1] 马静[1] 马芳萍 魏晓丽[2] 

机构地区：[1]延安市人民医院病理科,陕西延安716000 [2]延安大学医学院,陕西延安716000

出　　处：《现代肿瘤医学》2013年第11期2584-2586,共3页Journal of Modern Oncology

基　　金：延安市科学技术研究发展计划基金资助项目(编号:2011ks-07)

摘　　要：目的:将细胞DNA倍体定量分析方法与TBS常规细胞学方法应用于宫颈癌及癌前病变的筛查,比较两者的诊断效果,以提高诊断的准确性。方法:对门诊及体检的3165名妇女进行宫颈癌筛查。用宫颈刷取材,液基薄层制片后,分别进行DNA染色和巴氏染色,并分别进行全自动细胞扫描诊断和TBS常规细胞学诊断,对以上检查可疑的妇女在阴道镜下行宫颈组织学活检。结果:细胞DNA倍体分析系统发现异常303例(9.57%),常规细胞学检查发现异常189例(5.98%)。135例妇女进行了活检,以病理诊断结果为标准,计算出DNA定量分析方法及常规细胞学方法在筛查CINⅡ以上宫颈病变的敏感性、特异性和阳性预测值,分别为84.21%、86.96%、84.21%和46.97%、91.30%和83.78%。结论:细胞DNA倍体定量分析方法在宫颈癌及癌前病变的筛查中具有较高的敏感性。Objective：To evaluate values of combination application of DNA ploidy analysis and routine cytology examination in the liquid-based thin-layer cytology test for cervical squamous cell carcinoma.Methods：Cytology samples were collected by cervical brushing from 3,165 out-hospital patients or healthy women for cancer screening and prepared by liquid-based thin-layer technology.The ploidy status was evaluated by Fulgen reaction and DNA cytometry,and the routine examination was performed by Barth-staining and using the Bathesda system （TBS）.Biopsy was performed for the suspicious cases of neoplastic lesions.Results：DNA ploidy aberrations were found in 303 （9.57％）of the cases,and atypical squamous cells or more severe abnormalities were noted via routine cytological examination in 189（5.98％）of them.Biopsy results were retrieved for 135 cases,and sensitivity rate,specificity rate and prediction accuracies of these two approaches were 84.2％,87.0％,84.2％ and 47.0％,91.3％,83.8％,respectively.Conclusion：DNA cytometry is proven to be more sensitive than the routine cytological examination in the screening program for cervical squamous cell carcinoma and its precursors.

关 键 词：细胞DNA倍体定量分析 常规细胞学 癌前病变 

分 类 号：R737.33[医药卫生—肿瘤]