DNA氧化性损伤标志物8-羟基脱氧鸟苷在阿尔茨海默病和血管性痴呆患者血浆中的变化  

Variation of plasma oxidative DNA damage marker 8-hydroxy-2＇-deoxyguanosine in patients with Alzheimer＇s disease versus vascular dementia

作　　者：朱红敏[1] 章军建[1] 杨英[1] 

机构地区：[1]武汉大学中南医院神经内科,430071

出　　处：《中华老年医学杂志》2013年第11期1164-1168,共5页Chinese Journal of Geriatrics

基　　金：国家自然科学基金（30970962）

摘　　要：目的观察阿尔茨海默病（AD）与血管性痴呆（VaD）患者血浆中DNA氧化损伤标志物8-羟基脱氧鸟苷（8-OHdG）水平的变化，探讨DNA氧化损伤在AD和VaD发病中的作用。方法用酶联免疫吸附试验的方法测定35例AD患者和28例VaD患者以及与之年龄相匹配的23例健康者血浆中8-OHdG的水平，用简易智力量表（MMSE）评测AD和VaD患者的认知功能。结果AD组、VaD组、健康对照组患者血浆中8-OHdG浓度分别为（601．8±266．0）ng／L、（1748．0±665．6）ng／L和（352．0±94．5）ng／L，各组间血浆8-OHdG水平差异有统计学意义（P〈0．001）。VaD组患者血浆中8-OHdG水平与对照组比较差异有统计学意义（P〈0．05）；而AD组患者血浆中8-OHdG水平与对照组比较差异无统计学意义（P〉0．05）；并且VaD组与AD组比较，8-OHdG水平变化的差异也有统计学意义（P〈0．05）。各组血浆8-OHdG水平与年龄均呈正相关，分别为：AD组（r=0．661，P〈0．01）、VaD组（r=0．702，P〈0．01）、正常对照组（r=0．719，P〈0．01）。而各组血浆8-OHdG水平与教育水平无明显相关性（均P〉0．05）。在AD组和VaD组患者中根据其危险因素（性别、吸烟、高血压、糖尿病、高血脂）进行分组，比较各组间血浆8-OHdG水平的差异，结果发现各组问比较差异均无统计学意义（均P〉0．05）。校正了性别、年龄、教育水平、吸烟、高血压、高血脂、糖尿病因素的影响后，采用多分类Logistic回归分析结果显示：血浆中高水平8—0HdG在VaD组中比AD组和正常对照组的可能性均高。VaD组血浆8-OHdG水平与MMSE分数呈显著负相关性（r=0．592，P〈0．01）；AD组血浆8-OHdG水平与MMSE分数无相关性（r=0．122，P〉0．05）。结论VaD患者存在明显的DNA氧化损伤，其血浆DNA氧化损伤程度显著高于AD患者与健康者；VaD患者的血浆DNA氧化损伤标志物8-OHdG与其认知功能损害�Objective To observe the variation of plasma 8 bydroxy-2＇-deoxyguanosine （8-OHdG）, a biomarker of oxidative DNA damage, in patients with Alzheimers disease （AD） versus vascular dementia （VaD）, and to investigate the important role and the difference of oxidative DNA damage in the initiation and development of AD and VaD. Methods 35 AD patients, 28 VaD patients and 23 age and sex matched normal controls were enrolled in this study. Plasma 8-OHdG was measured by an enzyme-linked immunosorbent assay （ELISA） method. Cognition function in AD and VaD patients was measured by Mini-Mental State Examination （MMSE）. Results There were significant differences in plasma 8-OHdG concentrations among AD, VaD and control groups （601.84-266.0） ng/L, （1748.0±665.6）ng/L, （352.0±94.5）ng/L, respectively, P〈0.001]. Plasma 8-OHdG was significantly higher in VaD group than in control group （P〈O. 05）. While, there was no significant difference in plasma 8-OHdG concentration between AD group and control group （P〉 0.05）. Plasma 8-OHdG was significantly higher in VaD group than in AD group （P〈0.05）. Plasma 8-OHdG levels were positively related with age in AD, VaD and control groups （r=0. 661, 0. 702, 0. 719, respectively, all P〈0.01）. No correlations were found between plasma 8-OHdG concentration and education level among the three groups （all P〉0.05）. AD and VaD patients were subgrouped according to the risk factors including gender, smoking, hypertension, diabetes, hyperlipidemia. There were no significant differences in plasma 8-OHdG level between the subgroups （all P〉 0.05）. After adjusting for sex, age, education level, smoking, hypertension, hyperlipidemia and diabetes, logistic regression analysis showed that the probability of high plasma 8-OHdG level was higher in VaD group than in AD and control groups. Plasma 8-OHdG level had a negative correlation with MMSE score in VaD group （r=-0. 592, P〈0.01）, while no correlation was found between plasma 8-OHdG l

关 键 词：阿尔茨海默病 痴呆 血管性 氧化性应激 

分 类 号：R749.1[医药卫生—神经病学与精神病学]