丹参对大鼠离体保存再灌注肝脏IL-10mRNA的影响  

Effect of salviamiltiorrhiza on expression of IL-10 mRNA in isolated rat liver with preservation and reperfusion

作　　者：苏志良[1] 赵浩亮[2] 曹燕[1] 魏爱英[1] 

机构地区：[1]中国人民解放军二六四医院普外科,山西太原030001 [2]山西医科大学第一医院普外科,山西太原030001

出　　处：《中国现代普通外科进展》2013年第10期766-768,共3页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨丹参对大鼠离体保存后再灌注肝脏IL-10 mRNA的影响。方法:建立离体大鼠肝脏保存再灌注模型,应用RT-PCR方法检测UV液、UV液+丹参保存并再灌注的离体肝脏的IL-10 mRNA表达。结果:丹参保存16、24、32 h再灌注的大鼠离体肝脏IL-10 mRNA表达分别为68.55±5.37、49.73±3.74、32.65±2.39;明显高于对照组(37.62±3.37、23.70±2.35、13.71±1.28),差异有统计学意义(P<0.05)。结论:丹参能够增加肝脏保存后再灌注IL-10 mRNA表达,对肝脏保存再灌注损伤可能具有防治作用。objeetive：To investigate the effect of salviamiltiorrhiza on expression of IL-10 mRNA in isolated rat liver with preservation and reperfusion.Methods：The：model of isolated rat liver with preservation and reperfusion was set up,the expression of IL-10 mRNA was detected with RT-PCR in different preservation time of the isolated liver.Results：ln salv amiltiorrhiza-treated group,expression level of IL-10 mRNA was obviously higher than that in control group after preservation for 16、24、32 h.followed by same reperfusion time （6855 ±5.37 vs 37.62 ±3.37,49.73 ± 3.74 vs 23.70 ± 2.35,32.65 ± 2.39 vs 13.71 ± 1.28,P ＜ 005）.Conclusions：：Saiviamiltiorrhiza can increase expression of IL-10 mRNA to alleviate reperfusion injure of the isolated rat liver.

关 键 词：肝移植 再灌注损伤 丹参 白细胞介素-10 大鼠 

分 类 号：R575[医药卫生—消化系统] R617[医药卫生—内科学]