不同肺癌细胞株中VEGFR-2 mRNA表达的实验研究  

The expressions of VEGFR-2 mRNA in different lung cancer cell lines

作　　者：张莹[1] 蒋晓东[2] 乔云[2] 刘毅[2] 刘亮[2] 丁曼华[2] 

机构地区：[1]解放军第149医院消化肿瘤科,222042 [2]连云港市第一人民医院肿瘤科,222002

出　　处：《临床肿瘤学杂志》2013年第10期879-881,共3页Chinese Clinical Oncology

基　　金：国家自然科学基金资助项目(81272558);卫生部科研基金资助项目(W201210);江苏省自然科学基金资助项目(BK2012661);中华国际医学交流基金会资助项目(CIMF-F-H001-250)

摘　　要：目的研究不同非小细胞肺癌(NSCLC)细胞株中血管内皮生长因子受体-2(VEGFR-2)mRNA的表达情况。方法采用实时荧光定量PCR法检测人肺黏液表皮样癌NCI-H292细胞株、人肺腺癌NCI-H1299细胞株、人肺腺癌A549细胞株、人肺巨细胞癌95D细胞株和人肺鳞癌Calu-1细胞株中VEGFR-2 mRNA的表达。结果 Calu-1、NCI-H292、95D、NCI-H1299和A549细胞株中VEGFR-2 mRNA表达依次为1.213±0.134、1.008±0.164、0.754±0.088、0.582±0.171和0.121±0.026,差异有统计学意义(F=31.875,P=0.000)。Calu-1与NCI-H292细胞株间VEGFR-2 mRNA表达无统计学差异(P=0.080),NCI-H1299与95D细胞株间VEGFR-2 mRNA表达亦无统计学差异(P=0.137),其余细胞株间差异均有统计学意义(P均<0.05)。结论不同NSCLC细胞株中VEGFR-2 mRNA表达量不同,可能与不同肿瘤细胞的生物学行为有关。Objective To investigate the expressions of vascular endothelial growth factor receptor 2 （VEGFR-2） mRNA in different lung cancer cell lines.Methods The expressions of VEGFR-2 mRNA in the human pulmonary mucoepidermoid carcinoma cell line NCI-H292,lung adenocarcinoma cell line NCI-H1299,lung adenocarcinoma cell line A549,lung giant cell carcinoma 95D and lung squamous carcinoma cell line Calu-1 were detected by real-time quantitative PCR （RT-PCR）.Results The expressions of VEGFR-2 mRNA were 1.213 ± 0.134,1.008 ±0.164,0.754 ±0.088,0.582 ±-0.171 and 0.121 ±0.026 in Calu-1,NCI-H292,95D,NCI-H1299 and A549 cell lines,showing significant differences （F =31.875,P =0.000）.No significant difference was observed in the expression of VEGFR-2 mRNA between Calu-1 and NCI-H292 （P =0.080） or between NCI-H1299 and 95D （P =0.137）.Conclusion The expression of VEGFR-2 mRNA is diverse in different lung cancer cell lines,which is closed with the different biological behavior.

关 键 词：血管内皮生长因子受体2 非小细胞肺癌 MRNA 

分 类 号：R734.2[医药卫生—肿瘤]