长链非编码RNA在结肠癌中的表达研究  

Study on expression of long non-coding RNA in colon carcinoma

作　　者：曹伟军[1] 吴海露[1] 张予蜀[1] 陈震球[1] 袁捷[1] 张振玉[1] 

机构地区：[1]南京医科大学附属南京医院南京市第一医院消化内科,南京210006

出　　处：《临床肿瘤学杂志》2013年第10期882-886,共5页Chinese Clinical Oncology

摘　　要：目的探讨长链非编码RNA(lncRNA)在结肠癌中的表达情况。方法从公共数据平台Gene Expression Omnibus(GEO)下载结肠癌外显子芯片数据集GSE31737,包含40对结肠癌及癌旁组织。对外显子芯片探针进行重排,筛选出在基因水平特异性匹配lncRNA的探针。用Affymetrix Power Tools分析获得lncRNA表达谱,用LIMMA分析在结肠癌和癌旁组织中差异表达的lncRNA。结果共有136 053个探针特异性匹配9294个lncRNA,约包含了Ensembl数据库中76%的lncRNA。分析GSE31737发现,与癌旁组织相比,结肠癌组织中共有87个lncRNA差异表达,其中50个高表达,37个低表达。结肠癌与癌旁组织表达值倍数变化>1.5倍的基因共37个。结论 lncRNA表达在结肠癌组织中发生明显变化,有可能成为结肠癌的分子诊断和基因治疗的新靶点。Objective To analyze the expression of long non-coding RNA（lncRNA）in colon carcinoma.Methods We downloaded the publicly available human exon array GSE31737,which consisted of 40 paired colon carcinoma and normal adjacent tissue,from the Gene Expression Omnibus（GEO）.The probes of the human exon array were re-annotated and the probes uniquely mapping to lncRNAs at the gene level were retained.LncRNA expression profiles were generated by using Affymetrix Power Tools.The differentially expressed lncRNAs between colon carcinoma and normal adjacent tissue were analyzed by Bioconductor package LIMMA.Results We identified 136 053 probes uniquely mapping to lncRNAs at the gene level.These probes correspond to 9294 lncRNAs,covering nearly 76％ of lncRNAs in Ensembl.By analyzing GSE31737,87 lncRNAs were differentially expressed in colon carcinoma compared with normal adjacent tissue,among which 50 were up-regulated and 37 down-regulated.Thirty-seven lncRNAs showed more than 1.5-fold-change in gene expression.Conclusion LncRNA expression of colon carcinoma changes significantly in comparison with normal adjacent tissue.LncRNA may be used as a new candidate target for molecular diagnosis and gene therapy of colon carcinoma.

关 键 词：结肠癌 长链非编码RNA 基因芯片 

分 类 号：R735.3[医药卫生—肿瘤]