YAP2蛋白在涎腺腺样囊性癌组织及细胞中的表达  

Role of EGFR on Expression of YAP2 in Salivary Adenoid Cystic Carcinoma

作　　者：李莹[1] 王喜华[2] 于洋[2] 刘培红[2] 马肃[2] 赵尔飏[2] 

机构地区：[1]哈尔滨医科大学附属第二临床医院口腔科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属口腔医院牙周病科,黑龙江哈尔滨150001

出　　处：《口腔颌面外科杂志》2013年第5期357-360,共4页Journal of Oral and Maxillofacial Surgery

基　　金：黑龙江省教育厅科学技术研究资助项目(11541196)

摘　　要：目的:观察YAP2蛋白在涎腺腺样囊性癌组织及细胞中的表达。方法:通过免疫组织化学法检测YAP2蛋白在涎腺腺样囊性癌组织及细胞中的表达,用Western印迹法检测表皮生长因子受体(epidermal growth factor receptor,EGFR)对涎腺腺样囊性癌(salivary gland adenoid cystic carcinoma,SACC)SACC-83细胞YAP2蛋白的抑制。结果 :SACC组织中YAP2蛋白在肿瘤细胞胞质中阳性表达,正常腺体中YAP2蛋白主要表达在部分导管上皮中,两组相比差异有统计学意义(P<0.05)。免疫细胞化学染色结果显示SACC-83中YAP2蛋白阳性表达,加入EGFR蛋白后,SACC-83中YAP2蛋白表达有所降低。Western印迹法结果显示,不同质量浓度的EGFR蛋白(5、10、20、40μg/L)对SACC-83细胞YAP2蛋白的表达都有一定抑制作用,其中40μg/L质量浓度组抑制作用最明显。结论:YAP2蛋白在涎腺腺样囊性癌组织及细胞中高表达,EGFR蛋白对YAP2蛋白在SACC-83中表达有抑制作用。Objective： This study aimed to observe the expression of YAP2 in salivary gland adenoid cystic carcinoma tissue and cell line. Methods： YAP2 protein in salivary gland adenoid cystic carcinoma tissue and cells were identified by immunohistochemistry with a muhiclonal antibody. EGFR inhibition of YAP2 in SACC-83 cells was detected by western blotting. Results： In SACC, positive expression of YAP2 were in the cytoplasm. In normal salivary gland, YAP2 positive were identified in some duct epithelia. There was statistical significance between them （P〈0.05）. Expression of YAP2 protein in SACC-83 was down-regulated by the addition of EGFR. Western blot analysis showed EGFR（5, 10, 20, 40 p,g/L） inhibited the expression of YAP2 in SACC-83, and 40 p,g/L was most effective. Conclusion： YAP2 protein is overexpressed in salivary gland adenoid cystic carcinoma tissue and cells. EGFR protein inhibits the expression of YAP2 in the SACC-83.

关 键 词：YAP2 涎腺腺样囊性癌 SACC-83 表皮生长因子受体 

分 类 号：R739.87[医药卫生—肿瘤]