非小细胞肺癌患者肿瘤浸润性淋巴细胞中T细胞克隆性增殖  

THE FEATURE OF CLONAL EXPANSION AND DISTRIBUTION OF TCR Vβ T CELLS OF CULTURED NSCLC TUMOR-INFILTRATING LYMPHOCYTES

作　　者：杨学宁[1] 吴一龙[1] 李锦添[1] 王思愚[1] 周昕熙[1] 

机构地区：[1]中山医科大学肺癌研究中心,中山医科大学肿瘤防治中心胸科,广州510060

出　　处：《肿瘤》2000年第6期396-400,共5页Tumor

基　　金：卫生部科学研究基金! (98-2 -3 77) ;广东省科委攻关项目!(99M0 490 3 G)

摘　　要：目的　分析非小细胞肺癌 (non- small- cell lungcancer,NSCL C)病人肿瘤浸润性淋巴细胞 (tum or- infiltrat-ing lymphocytes,TIL)的 T细胞受体 (TCR) Vβ2 4个亚家族基因的克隆性增殖及分布情况。方法　肺腺癌病人 1例 ,术中取新鲜肺癌组织 ,肺癌组织经处理后分别与自身肿瘤细胞共同培养 3.5天和 7天。 10例健康人、T细胞株 Jurkat和 B细胞株 Raji作为对照。标本共 16份 ,提取 m RNA后经反转录多聚酶链反应 (RT- PCR) ,PCR产物进一步经基因扫描分析 Vβ2 4个亚家族基因的互补决定区 3(CDR3)以确定 T细胞克隆性。结果　 NSCL C病人外周血 T细胞仅表达 16个Vβ亚家族 ,Vβ6和 Vβ17呈寡克隆。新鲜肺癌组织 TIL仅检测到 Vβ19表达 ,呈寡克隆 ;培养 7天后 ,检测 7个 Vβ亚家族表达 ,Vβ8和 Vβ16呈寡克隆。 10例正常人 T细胞表达所有 2 4个 Vβ亚家族且均呈多克隆性分布。结论　 NSCL C病人 TIL中 ,TCR Vβ2 4个亚家族存在倾斜性分布 ,T细胞存在克隆性增殖。这可能是肿瘤相关抗原 (tumor associatedantigens,TAA)刺激引起的特异性 T细胞免疫反应。研究结果提示 ,构成经培养的 TIL 的主要 T细胞成分是克隆性增殖的表达有限几个 TCR Vβ亚家族的 T细胞 ,这些克隆性增殖 T细胞可能是 TIL 对自身肿瘤细胞具有?Objective To study the distribution of TCRVβ subfamilies and clonal expansion of peripheral blood lymphocytes (PBL) and tumor-infiltrating lymphocytes (TIL) in non-small-cell lung cancer (NSCLC) patients.Methods Fresh NSCLC tumor samples were collected soon after the resection, and the TILs were cultured for 0,3.5,7 days respectively. PBL collected before operation. The complementarity determine region 3 (CDR3) of 24 TCRVβ subfamilies genes were amplified by RT-PCR, the PCR products were further analyzed by Genescan to identify the clonality of the T cells.Results PBL expressed 16 TCRVβ subfamilies, Vβ6 and Vβ17 were oligoclonal. Fresh TIL expressed 1 TCRVβ subfamily, Vβ19 was oligoclonal. TIL cultured 3.5 days expressed 5 TCRVβ subfamilies, Vβ5 was biclonal, Vβ16 was oligoclonal. TIL cultured 7 days expressed 7 TCRVβ subfamilies, Vβ8 and Vβ16 were oligoclonal. All 24 TCRVβ subfamilies were expressed and polyclonal in normal control.Conclusion The skew distribution and clonal expansion of TCRVβ subfamily T cells were detected in PBL and cultured NSCLC TILs. It may be the T cell clones for specific immune response due to tumor-associated-antigens(TAA). The present result showed that the cultured TIL mainly consists of a few TCRVβ subfamily clonal expanded T cell subsets. It suggests that these clonal expansion T cells are potentially specific cytotoxic to autologous tumor cells.

关 键 词：非小细胞肺癌 肿瘤浸润性淋巴细胞 T细胞 

分 类 号：R734.2[医药卫生—肿瘤]