榄香烯诱导HeLa细胞凋亡的实验研究  

Experimental Study on the Elemene Emulsion-Induced Apoptosis of HeLa Cell

作　　者：马东礼[1] 肖家祁[1] 童善庆[1] 胡宝瑜[1] 侍庆[2] 胡烈薇[2] 

机构地区：[1]上海第二医科大学院微生物学教研室，上海 200025 [2]瑞金医院妇产科

出　　处：《上海第二医科大学学报》2000年第6期484-487,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金资助项目(39730270)

摘　　要：目的从 8种抗病毒、抗肿瘤药物中筛选出诱导HeLa细胞凋亡的药物 ,并对其抗肿瘤机理进行探讨。方法应用相差显微镜、电镜和流式细胞仪等检测手段 ,证实HeLa细胞凋亡 ,并对凋亡的HeLa细胞周期变化进行分析。　结果榄香烯阻滞HeLa细胞在G2 /M期 ,降低HeLa细胞分裂能力 ,抑制其增殖 ;榄香烯抗肿瘤作用存在明显的阈值 ,即达到某一有效剂量 ,就可发挥强烈的抗肿瘤作用。　结论榄香烯是一种有效的诱导细胞凋亡的药物 ,对指导临床药物治疗宫颈癌具有重要意义。Objective In this study, with fluorescence stain, we screened out a drug that could induce apoptosis of the HeLa cell line from eight antivirus and antitumor drugs, and the mechanism of apoptosis of HeLa cells induced by the drug was analyzed. Methods The apoptosis of HeLa cells has been confirmed by means of light microscopy, electron microscopy and flow cytometry. In addition, we also studied on the change of HeLa cell ratio in the proliferation cycle induced by the drug. Results Results showed that elemene emulsion could arrest HeLa cell growth in G 2/M phrase, and inhibit the proliferation of HeLa cells. Elemene emulsion is dosage-dependent in the inducing apoptosis of Hela cells. Once it exceeds the effctive dosage, it can play a strong role in inhibiting tumors. Conclusion Elemene emulsion is an effective drug which may induce the apoptosis of HeLa cells. It may be worthy to be adapted in directing clinical cervical cancer therapy.

关 键 词：榄香稀 凋亡 宫颈癌 HeLe 细胞凋亡 实验研究 

分 类 号：R737.33[医药卫生—肿瘤]