凋亡调控基因Bcl-2/BaxmRNA在子宫平滑肌瘤中的表达  

Study on the Expression of Apoptosis-Controlled Gene Bcl-2/Bax mRNA in the Uterine Leiomyoma

作　　者：李卫平[1] 严隽鸿[1] 林其德[1] 裴军[1] 丁传伟[1] 

机构地区：[1]上海第二医科大学仁济医院妇产科,上海200001

出　　处：《上海第二医科大学学报》2000年第6期514-517,共4页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：目的探讨细胞凋亡调控基因Bcl- 2 /BaxmRNA在子宫肌瘤中的表达及与ER、PR的关系。　方法应用免疫组化法测定子宫肌瘤内ER、PR的含量 (2 8例 )。用RT -PCR法测定肌瘤内Bcl- 2 /BaxmRNA (2 1例 )。以肌瘤邻近的正常子宫平滑肌作对照。　结果子宫肌瘤内ER、PR ,Bcl- 2 /Bax比值显著高于正常子宫平滑肌 (P<0 .0 5 和P<0 .0 1) ;肌瘤ER、PR与Bcl- 2 /BaxmRNA比值呈正相关。　结论细胞凋亡调控基因Bcl- 2 /Bax可能参与子宫肌瘤的发生 ;而雌、孕激素与ER、PR结合刺激肌瘤生长则可能与影响这一基因的表达有关。Objective To investigate the expression of apoptosis-controlled gene Bcl-2/Bax mRNA in leiomyoma and its relationship with ER、PR. Methods To measure ER, PR volume (28 cases) in leiomyoma and the ratio of Bcl-2/Bax mRNA (21 cases) by immunochemistry and RT-PCR, respectively, and use the myometrium as control. Results The content of ER, PR and the ratio of Bcl-2/Bax mRNA in leiomyoma were significantly higher than those in the myometrium (P<0.05 and 0.01); there was positive correlation for ER, PR versus ratio of Bcl-2/Bax mRNA in leiomyoma. Conclusion Apoptosic-controlled gene Bcl-2/Bax may play a role in the pathogenesis of leiomyoma; the stimalating effect of estrogen and progesterone combining with ER, PR on leiomyoma may be related with their regulation of the expression of Bcl-2/Bax mRNA.

关 键 词：子宫平滑肌瘤 细胞凋亡 BCL-2/BAX 雌激素受体 孕激素受体 

分 类 号：R737.33[医药卫生—肿瘤]