不同贮藏温度下杉木种子的生理生化特征  

Physiological and Biochemical Characteristics of Cunninghamia lanceolata under Didderent Storage Temperatures

作　　者：陈琴[1] 黄开勇[1] 戴俊[1] 郝海坤[1] 蓝肖[1] 

机构地区：[1]广西壮族自治区林业科学研究院,广西南宁530002

出　　处：《安徽农业科学》2013年第22期9355-9357,共3页Journal of Anhui Agricultural Sciences

基　　金：广西林业科技项目"杉木良种选育及高效栽培技术研究与示范"(桂林科字[2010]3号);广西地方标准制修订项目"杉木容器育苗技术规程"(2011-1)

摘　　要：[目的]研究贮藏温度对贮藏过程中杉木种子活力的影响。[方法]采用生理生化检测方法,测定不同贮藏温度下杉木种子细胞膜透性、丙二醛含量、可溶性糖含量以及超氧化物歧化酶和过氧化氢酶活性。[结果]细胞膜透性在不同贮藏温度间存在显著差异,贮藏温度为4℃时,细胞膜渗透率最小,其次是-80℃;丙二醛含量在贮藏温度为40℃时显著高于其他温度处理,但在其他温度处理间无显著差异;超氧化物歧化酶和过氧化氢酶活性在不同贮藏温度间均存在显著差异,贮藏温度为4℃时,2种酶的活性均最强;可溶性糖含量在不同贮藏温度间无显著差异;细胞膜透性、丙二醛含量分别与超氧化物歧化酶之间存在极显著和显著的负相关。[结论]该试验条件下4℃是杉木种子的最适贮藏温度。[ Objective ] Study the effects of storage temperatures on Cunninghamia lanceolata seeds vigor during storage. [ Method ] In this paper, cell membrane permeability, Malondiadehyde contents, soluble sugar contents, Superoxide dismutase activity and Catalase activity of Cunninghamia lanceolata seeds under different storage temperatures were determined by using physiological and biochemical detection meth- ods. [ Result] There were significant differences of cell membrane permeability among different storage temperatures. At the storage tempera- ture of 4 ℃, the cell membrane permeability was the lowest, followed by - 80 ℃. The content of Malondiadehyde at the storage temperature of 40 ℃ was significant higher than other temperatures, but it showed no significant differences among the other four temperatures. Obvious differences of Superoxide dismutase activity and Catalase activity were observed on different temperatures, and the highest activity of this two enzymes were found at the storage temperature of 4 ℃ respectively. There was no significant differences of the contents of soluble sugar on dif- ferent storage temperatures. Very significant negatively correlation was found between cell membrane permeability and Superoxide dismutase activity, and significant negatively correlation was found between Malondiadehyde contents and Superoxide dismutase activity. [ Conclusion] 4 ℃ is the optimal storage temperature for C. lanceolata seeds.

关 键 词：贮藏温度 杉木 种子 生理生化特征 

分 类 号：S791[农业科学—林木遗传育种]