一种多酚氧化酶提取新方法与几种常规方法的比较研究  

Study on A New Method for Extracting Polyphenol Oxidase Compared with Conventional Methods

作　　者：毕云枫[1] 姜仁凤[1] 刘薇薇[1] 李彦阳[1] 沈明浩[1] 

机构地区：[1]吉林农业大学食品质量与安全实验室,吉林长春130118

出　　处：《安徽农业科学》2013年第22期9418-9420,共3页Journal of Anhui Agricultural Sciences

基　　金：吉林省科技厅项目(20090553);吉林省教育厅项目(吉教科合字[2011]第35号)

摘　　要：[目的]对一种新的多酚氧化酶的提取方法与几种常用提取方法的综合效果进行比较。[方法]以苹果梨为研究对象,对匀浆法、匀浆吸附法、匀浆后丙酮抽提法、丙酮粉法、丙酮抽提后吸附法这5种多酚氧化酶提取方法所得酶液进行了多酚类化合物残余、总酶活及酶的稳定性的比较。同时,还对几种氨基酸对酪氨酸酶的抑制情况进行了检测。[结果]试验得出,以改进的丙酮抽提后吸附法所得酶液多酚残余少,总活力保持良好,为几种方法中最佳的提取方法。谷胱甘肽、谷氨酰胺、半胱氨酸、谷氨酸对酪氨酸酶的活力均有抑制作用,其IC50值分别为(0.48±0.028)、(0.59±0.032)、(0.37±0.016)和(1.87±0.087)mmol/L。[结论]研究可为多酚氧化酶的相关研究提供参考。[ Objective] A new method for extracting polyphenol oxidase was compared with several conventional methods. [ Method] Using hom- ogenization method, homogenization adsorption method, homogenization post-acetone extraction method, acetone powder method, acetone ex- traction post-adsorption method to have extraction of polyphenol oxidase from Pyrus bretschneideri Rehder. And the results of absorbency, total activity and stability were compared. [ Results ] The results showed that acetone extraction post-adsorption method was the best in the five methods. The enzyme solution obtained by acetone extraction post-adsorption method was the remain of polyphenolic compounds were little and maintaining good the total activity of polyphenol oxidase. Glutataione, glutamine, cysteine and glutamate on each polyphenol oxidase activity were the inhibitory effects. The Icso of 4 kinds of amino acids were （0.48 ± 0. 028 ）, （0.59 ±0.032 ）, （0.37 ±0.016 ） and （ 1.87 ±0.087 ） mmol/L, respectively. [ Conclusion] The stduy can provide reference for relavant research of polyphenol oxidase.

关 键 词：多酚氧化酶 提取方法 总活力 稳定性 

分 类 号：S188.3[农业科学—农业基础科学]