硝酸铅对绿豆苗的毒性研究  

Toxic Effects of Lead Nitrate on Mung bean

作　　者：宋志慧[1] 翟胜[1,2] 

机构地区：[1]青岛科技大学环境与安全工程学院,山东青岛266042 [2]天津市安全生产技术研究中心,天津300381

出　　处：《安徽农业科学》2013年第22期9385-9387,共3页Journal of Anhui Agricultural Sciences

基　　金：山东省社会科学规划项目(03CJZ03);山东省高等学校实验技术研究项目(2005-188)

摘　　要：[目的]探讨硝酸铅对绿豆苗的毒性。[方法]以绿豆苗为试验材料,通过研究不同浓度(0、100、200、400、600 mg/L)的硝酸铅对绿豆苗生长发育及多酚氧化酶活性、过氧化氢酶活性、叶绿素含量的影响,初步探讨硝酸铅对绿豆苗的毒性作用。[结果]绿豆苗培养5 d后,硝酸铅对绿豆苗叶和根的IC50分别为276.33、6.47 mg/L;随着硝酸铅浓度的升高,绿豆芽叶中PPO活性表现为抑制-诱导效应,绿豆芽根中PPO活性表现为诱导-抑制效应;绿豆芽叶和根中CAT活性表现为诱导-抑制效应;绿豆芽叶中的叶绿素含量表现为抑制效应。[结论]该研究为及时发现并排除重金属铅污染提供了理论和直观依据。[ Objective] The study aimed to discus the toxic effects of lead nitrate on mung bean. [ Method] Toxic effects of different concen- trations of lead nitrate （0, 100,200,400 and 600 mg/L） on the growth, polyphenol oxidase activity, catalase activity and chlorophyll content of mung bean were analyzed. [ Result] After mung bean seedings were cultured for 5 d, the IC50 of lead nitrate for both leaves and roots was 276.33 and 6.47 mg/L respectively. With the increase of lead r^itrate, the PPO activity of mung bean seedings decreased firstly and then in- creased in leaves, while it rose firstly and then reduced in roots ; the CAT activity of mung bean seedings enhanced firstly and then dedlined in both leaves and roots; the chlorophyll content showed an inhibitory effect. [ Conclusion] The research could provide theoretical and intuitive bases for discovering and getting rid of lead pollution opportunely.

关 键 词：硝酸铅 绿豆苗 IC50 PPO活性 CAT活性 叶绿素含量 

分 类 号：S181.3[农业科学—农业基础科学] X503.2[环境科学与工程—环境工程]