H9N2禽流感不同毒株鸡胚交叉中和指数及其与HA基因氨基酸同源性相关的研究  

Relationship between the Neutralization Index in Chicken Embryo and the Homologies of HA Gene of Different H9N2 AIV Isolates

作　　者：刘娟[1] 张伟[2] 徐怀英[2] 马秀丽[2] 李建[1] 黄兵[2] 秦卓明[1,2,3] 崔言顺[1] 

机构地区：[1]山东农业大学,山东泰安271018 [2]山东农业科学院,山东济南250100 [3]山东省健牧生物药业,山东济南250100

出　　处：《华北农学报》2013年第5期23-28,共6页Acta Agriculturae Boreali-Sinica

基　　金：山东省科技攻关重点支持项目(2009GG10009006);济南市成果转化支持项目(2012CG92)

摘　　要：对国内1998-2011年流行的22株H9N2分离株进行病原鉴定和HA基因的序列测定,结合在GenBank中公开的46株国内外H9N2病毒进行序列比对,绘制系统发育树。选取在时间、地点和遗传进化方面具有代表性意义的6株H9N2毒株,分别制备单因子血清,进行鸡胚交叉中和试验,计算不同毒株之间的鸡胚交叉中和指数,确定H9N2不同毒株之间的抗原相关系数,并与其HA基因氨基酸的同源性进行相关比较。以期在分子水平上探讨我国疫苗免疫压力下H9N2禽流感病毒的变异,研究病毒的抗原性变化,探讨HA基因变异对抗原性的影响。结果表明:鸡胚中和指数与HA氨基酸同源性呈显著相关(P<0.01,r=0.639 8),表明在多年的疫苗免疫压力下,H9N2病毒已经在分子水平和抗原性上发生了一定程度的变异。该结果为研制新型的H9N2疫苗提供理论支持。In this paper ,22 prevailed H9 N2 AIV isolates in China from 1998 to 2011 were identified ,purified , sequenced,analyzed,and compared with the 46 H9N2 references in GenBank.Then,the phylogenetic tree was re-constructed based on those H9N2 strains.First of all,6 representative isolates were chosen ,which belong to different genotype ,to make for signal positive serum .Secondly ,the cross neutralization test was determined by using positive serums in specific pathogen free（SPF）chicken embryos,respectively.Thirdly,the correlation of the virus neutraliza-tion index（VN）in chicken embryo was compared with the amino acid homologies of HA gene of different H 9N2 iso-lates by SPSS 14 .0 software in order to explore the relationship between antigenic variations and genetic evaluations of the prevailed H9 N2 isolates undergo the effect of vaccination .Importantly ,the correlation coefficient and index of VN and amino acid homology was significantly correlated （P〈0.01,r=0.639 8）,which suggested that the H9N2 virus undergo more than ten years of vaccination ,has changed to some degree at the molecular level and antigenici-ty.Collectively,these data implied that antigenic variations of H 9N2 isolates result in the genetic mutations of HA , which was essential for developing the new vaccine .

关 键 词：H9N2 HA基因 抗原性 相关性 

分 类 号：Q78[生物学—分子生物学]