接合物蛋白在甲状腺乳头状微小癌中的表达及其临床意义  

Expression and Clinical Significance of CRKL in Thyroid Papillary Micro- carcinoma

作　　者：杨香山[1] 程绍梅[2] 郭京艳[3] 李成军[4] 李爱萍[5] 韩琳[5] 

机构地区：[1]山东省医学科学院附属医院病理科,山东省济南市250031 [2]山东省医学科学院附属医院妇科,山东省济南市250031 [3]山东省医学科学院附属医院药剂科,山东省济南市250031 [4]山东省医学科学院附属医院普外科,山东省济南市250031 [5]山东省医学科学院附属医院内科,山东省济南市250031

出　　处：《中国全科医学》2013年第29期3444-3446,3449,共4页Chinese General Practice

摘　　要：目的探讨接合物蛋白(CRKL)在甲状腺乳头状微小癌中的表达及其临床意义。方法收集山东省医学科学院附属医院2008—2013年经手术治疗的50例直径>1 cm的甲状腺乳头状癌患者、50例甲状腺乳头状微小癌患者、50例结节性甲状腺肿患者、50例正常者甲状腺组织的石蜡标本。采用免疫组织化学(SP)法与Western blotting实验检测CRKL的表达情况。结果甲状腺乳头状癌组、甲状腺乳头状微小癌组、结节性甲状腺肿组及正常甲状腺组织组CRKL的表达阳性率和CRKL表达的平均吸光度(MOD)比较,差异均有统计学意义(P<0.05);其中甲状腺乳头状癌组、甲状腺乳头状微小癌组高于结节性甲状腺肿组和正常甲状腺组织组(P<0.05)。CRKL表达量与甲状腺乳头状癌和甲状腺乳头状微小癌患者年龄、性别、病灶数目均无关(P>0.05),与有无侵犯被膜、有无颈淋巴结转移有关(P<0.05)。结论 CRKL在直径>1 cm的甲状腺乳头状癌和甲状腺乳头状微小癌中均呈高表达,且与两者的颈淋巴结转移有关,临床均需要进行淋巴结清扫。Objective To explore the expression of CRKL in thyroid papillary micro - carcinoma and to discuss its clinical significance. Methods 50 cases of thyroid papillary carcinoma with diameter 〉 1 cm, 50 cases of thyroid papillary mi- cro - carcinoma, 50 cases of nodular goiter and 50 cases of normal thyroid tissues were collected from Shandong Academy of Med- ical Sciences Affiliated Hospital from 2008 to 2013. Immunohistochemistry SP method and Western blotting method were used to detect the expression of CRKL. Results The positive rate of CRKL expression and MOD in thyroid papillary carcinoma, thyroid papillary micro - carcinoma, nodular goiter and normal thyroid tissues were significantly different from each other （ P 〈 0. 05 ）. The positive rate of CRKL expression and MOD in thyroid papillary carcinoma and thyroid papillary micro - carcinoma were signif- icantly higher than nodular goiter and normal thyroid tissues （ P 〈 0. 05 ）. CRKL expression was not associated with age, gender and tumor number （ P 〉 0. 05）. Significant difference was found between CRKL expression and metastasis, violation of capsula （ P 〈 0. 05 ）. Conclusion CRKL is highly expressed in thyroid papillary carcinoma with diameter 〉 1 em and thyroid papillary micro -carcinoma, and is closely related to metastasis. Therefore, it is necessary to dissect lymph nodes.

关 键 词：甲状腺肿瘤 乳头状瘤 接合物蛋白 

分 类 号：R736.1[医药卫生—肿瘤]