高原低氧环境下结缔组织生长因子在正常和不同病变胃黏膜中的表达  

The Expression of Connective Tissue Growth Factor in Different Gastric Mucosa in Plateau

作　　者：祁永清 李菊英[2] 贺菊香[2] 王海燕[2] 

机构地区：[1]青海省西宁市第二人民医院外科,810001 [2]青海大学医学院病理教研室

出　　处：《中国全科医学》2013年第30期3562-3564,共3页Chinese General Practice

基　　金：青海大学医学院中青年科研基金项目(2007-ky-004)

摘　　要：目的探讨高原低氧环境下结缔组织生长因子(CTGF)在正常和不同病变胃黏膜中的表达。方法收集2007年7月—2008年3月青海大学附属医院行胃镜检查并符合慢性萎缩性胃炎的患者20例(其中慢性萎缩性胃炎肠上皮化生12例,非化生性慢性萎缩性胃炎8例),胃癌切除标本50例,体检时行胃镜检查并本人自愿情况下钳取的正常胃黏膜10例。采用免疫组织化学染色法观察CTGF的表达情况。结果正常胃黏膜中CTGF阳性表达1例(1/10);慢性萎缩性胃炎肠上皮化生组织中CTGF阳性表达11例(11/12);非化生性慢性萎缩性胃炎组织中CTGF阳性表达4例(4/8);胃癌组织中CTGF阳性表达32例(64%),差异有统计学意义(χ2=16.097,P=0.0011)。慢性萎缩性胃炎肠上皮化生、胃癌组织中CTGF阳性表达率较正常胃黏膜中升高,差异均有统计学意义(χ2值分别为14.673和9.818,P值分别为0.0001和0.0017)。慢性萎缩性胃炎肠上皮化生、非化生性慢性萎缩性胃炎、胃癌组织中CTGF阳性表达率比较,差异均无统计学意义(P>0.0083)。结论在高原低氧环境下慢性萎缩性胃炎肠上皮化生和胃癌组织中CTGF的阳性表达增强,检测CTGF可以用于胃癌的癌前病变的筛选,提高了对胃癌发病的认识,而且对胃癌的预防、早期诊断及治疗有着重要的意义。Objective To investigate the expression of Connective Tissue Growth Factor （CTGF） in normal and pathological gastric mucosa in plateau. Methods 20 cases with chronic atrophic gastritis admitted to Qinghai University Affiliated Hospital from July 2007 to March 2008 were collected, including 12 cases with intestinal metaplasia and 8 cases without intestinal metaplasia. 50 samples of gastric cancer tissues and 10 samples of normal gastric mueosa were also collected. Immunohistochemistry method was used to detect the expression of CTGF. Results In normal gastric mucosa group, one case （1/10） had positive ex- pression of CTGF. In chronic atrophic gastritis with intestinal metaplasia group, 11 cases （11/12） had positive expression of CTGF, while 4 cases （4/8） had positive expression in those without intestinal metaplasia. In gastric cancer group, 32 cases （64. 0% ） had positive expression. The difference between the groups was statistically significant （X2 = 16. 097, P = 0. 0011 ）. The positive expression of CTGF in chronic atrophic gastritis with intestinal metaplasia group and gastric cancer group was signifi- cantly higher than that in normal gastric mucosa group （X2 = 14. 673, 9. 818 ; P =0. 0001, 0. 0017） . The positive expression of CTGF in chronic atrophic gastritis with intestinal metaplasia group, without intestinal metaplasia group and gastric cancer group showed no statistically significant difference （ P 〉 0. 0083 ） . Conclusion In high altitude and hypoxia environment, CTGF has higher level expression in gastric cancer and chronic atrophic gastritis. CTGF can be used for the detection of precancerous lesions of gastric cancer screening; not only does it increase the awareness of the incidence of gastric cancer, but it is also of great im- portance for the gastric cancer prevention.

关 键 词：高原病 缺氧 胃炎 胃肿瘤 结缔组织生长因子 

分 类 号：R735.2[医药卫生—肿瘤] R573.3[医药卫生—临床医学]